Hydration of actinocin derivative and its non and γ-irradiated complexes with DNA on data of ENF dielectrometry

  • O. V. Khorunzhaya Institute for Radiophysics and Electronics of NAS of Ukraine
  • V. A. Kashpur
  • A. A. Krasnitskaya
  • V. Ya. Maleev
Keywords: actinocin derivative, complex DNA with actinicin derivative, hydration, γ-irradiation, ENF differential dielectrometric method, electrophoresis

Abstract

Measurements of complex dielectric permittivity of aqueous salt solutions (0,08 M NaCl) of actinocin derivative with two methylene groups in aminoalkyl side chains (ActII) were carried out by a method of EHF differential dielectrometry. Dielectric properties of aqueous salt solutions of non and γ-irradiated at dose 800 Gy complexes of DNA-ActII (P/D=5) also were investigated. The value of gydration h (a number of water molecules per one molecule of ligand or DNA nucleotide) was calculated for all investigated substances. It was found that h=22 for ActII, h>30 for the complex DNA-ActII. γ-irradiation of the complex leads to the decrease of h by 18%. The degree of DNA fragmentation was determined by a method of electrophoresis. It was found that the irradiated complex DNA-ActII is more mobile and more fragmented than the non irradiated one.

 

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Author Biography

O. V. Khorunzhaya, Institute for Radiophysics and Electronics of NAS of Ukraine

12 Akad. Proskura St., Kharkiv, 61085, Ukraine

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Published
2018-06-25
Cited
How to Cite
Khorunzhaya, O. V., Kashpur, V. A., Krasnitskaya, A. A., & Maleev, V. Y. (2018). Hydration of actinocin derivative and its non and γ-irradiated complexes with DNA on data of ENF dielectrometry. Biophysical Bulletin, 1(12), 20-23. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12060
Issue
Vol. 1 No. 12 (2003): Біофізичний вісник
Section
Molecular biophysics

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