@article{Khorunzhaya_Kashpur_Krasnitskaya_Maleev_2018, title={Hydration of actinocin derivative and its non and γ-irradiated complexes with DNA on data of ENF dielectrometry}, volume={1}, url={https://periodicals.karazin.ua/biophysvisnyk/article/view/12060}, abstractNote={<p>Measurements of complex dielectric permittivity of aqueous salt solutions (0,08 M NaCl) of actinocin derivative with two methylene groups in aminoalkyl side chains (ActII) were carried out by a method of EHF differential dielectrometry. Dielectric properties of aqueous salt solutions of non and&nbsp;γ-irradiated at dose 800 Gy complexes of DNA-ActII (P/D=5) also were investigated. The value of gydration h (a number of water molecules per one molecule of ligand or DNA nucleotide) was calculated for all investigated substances. It was found that h=22 for ActII, h&gt;30 for the complex DNA-ActII.&nbsp;γ-irradiation of the complex leads to the decrease of h by 18%. The degree of DNA fragmentation was determined by a method of electrophoresis. It was found that the irradiated complex DNA-ActII is more mobile and more fragmented than the non irradiated one.</p> <h1>&nbsp;</h1&gt;}, number={12}, journal={Biophysical Bulletin}, author={Khorunzhaya, O. V. and Kashpur, V. A. and Krasnitskaya, A. A. and Maleev, V. Ya.}, year={2018}, month={Jun.}, pages={20-23} }