TY - JOUR AU - Khorunzhaya, O. V. AU - Kashpur, V. A. AU - Krasnitskaya, A. A. AU - Maleev, V. Ya. PY - 2018/06/25 Y2 - 2024/03/28 TI - Hydration of actinocin derivative and its non and γ-irradiated complexes with DNA on data of ENF dielectrometry JF - Biophysical Bulletin JA - Bioph. Bull. VL - 1 IS - 12 SE - Molecular biophysics DO - UR - https://periodicals.karazin.ua/biophysvisnyk/article/view/12060 SP - 20-23 AB - Measurements of complex dielectric permittivity of aqueous salt solutions (0,08 M NaCl) of actinocin derivative with two methylene groups in aminoalkyl side chains (ActII) were carried out by a method of EHF differential dielectrometry. Dielectric properties of aqueous salt solutions of non and γ-irradiated at dose 800 Gy complexes of DNA-ActII (P/D=5) also were investigated. The value of gydration h (a number of water molecules per one molecule of ligand or DNA nucleotide) was calculated for all investigated substances. It was found that h=22 for ActII, h>30 for the complex DNA-ActII. γ-irradiation of the complex leads to the decrease of h by 18%. The degree of DNA fragmentation was determined by a method of electrophoresis. It was found that the irradiated complex DNA-ActII is more mobile and more fragmented than the non irradiated one.  ER -