A comparative study of the growth of primary cultured skin and lung fibroblasts from rats of different ages
Abstract
Comparative study of viability and features of growth under the same conditions of cultivation of fibroblasts of derma and lungs from rats at the age of 0,5; 1; 3; 12; 24 and 30 months has been conducted. It has been shown that curves of growth of cells quantity for both types of fibroblasts are qualitatively similar. Dependence of quantity of proliferating cells on age of animals has a bell shape with a maximum in 3 months. In both cases this maximum is observed at the end of an exponential growth phase. With age of animals proliferative activity of both types of fibroblasts decreases, that is observed in decreased level of cell adhering, of quantity increase and density of culture and increase of quantity of apoptotic and "aged" cells in cultures at the end of an exponential growth phase. In the cell culture of lung fibroblasts this decline in the ability to proliferation is expressed statistically more sharply, than in the cell culture of dermal fibroblasts.
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