Change of cytoskeleton actin isoforms spectrum in normal and modified by TGF-β1 fibroblasts at mechanical deformation
Abstract
The dynamics of actin isoforms spectrum in normal deformed lung fibroblasts and fibroblasts modified by the action of TGF-β1 has been investigated. It has been shown that mechanical deformation leads to the appearance of α-actin isoform not detectable in non-deformed fibroblasts. It has been also shown that increase of α2β1 integrins on the surface of modified by TGF-β fibroblasts leads to the appearance of α-actin isoform at an earlier stage at the deformation of cells.
Downloads
References
Протокол 1. Электронный ресурс. (http://www.chemicell.com/products/fluorescent/index.html)
Протокол 2. Actin Cytoskeleton and Focal Adhesion Staining Kit. Электронный ресурс.
Протокол 3. Электронный ресурс.
(http://www.microscopyu.com/articles/fluorescence/filtercubes/dual/fitctritc/fitctritcindex.html)
Протокол 4. Изоэлектрофокусирование. Электронный ресурс. (http://195.178.207.228/work/FILES/19/2D-PAGE.pdf)
Сhemicell.com. Электронный ресурс.
(http://www.chemicell.com/products/Magnetic_Nanoparticle/Magnetic_Nanoparticles.html)
Cláudia A.C., Lamano-Carvalho T.L., Lacerda S.A. Biocompatibility of natural latex implanted into dental alveolus of rats // Journal of Oral Science. – 2006. – Vol.48, №4. – P. 201–205.
Freshney R.I. Culture of animal cells: a manual of basic technique and specialized. – Wiley-Blackwell Inc., 2010. – P.163.
Hwal Sun, Jong-Eun Lee Behavior of fibroblasts on a porous hyaluronic acid incorporated collagen matrix // Yonsei Medical Journal. – 2002. – Vol.43, №2. – P. 193–202.
Phelan M.C. Basic techniques for mammalian cell tissue culture // In: Current protocols in cell biology. – John Wiley & Sons Inc., 1998. – P. 82–83.
Rittié L., Fisher G.J. Isolation and culture of skin fibroblasts // Methods in Molecular Medicine. – 2005. –Vol.117. – P. 83–98.
Rubenstein P. Actin microheterogeity in chick embryo fibroblast // Proc. Natl. Acad. Sci. USA. – 1977. – Vol.74, №1. – P.120.
Sakiyama S., Fujimura S., Sakiyama H. Absence of γ-actin expression in the mouse fibroblast cell line // JBC. – 1981. – Vol.256, №1. – P.31.
Streuli C.H., Schmidhauser C., Kobrin M. et al. Extracellular matrix regulates expression of the TGF/~I gene Gene // The Journal of Cell Biology. – 1993. - Vol. 120. – № 1. – Р.253-260.
Thannickal V.J., Lee D.Y., White E.S. et al. Myofibroblast differentiation by transforming growth factor-β1 is dependent on cell adhesion and integrin signaling via focal adhesion kinase // The Journal of Biological Chemistry. – 2003. – Vol.278, №14. – P. 12384–12389.
Tomasek J.J. Gabbiani G., Hinz B. et al. Myofibroblast and mechanoregulation of connective tissue remodeling // Molecular Cell Biology. – 2002. – Vol.3. – P.352.
Vendra K.V., Wu Lin, Krishnan S. Polymer thin films for biomedical application // Nanomaterials for the Life Sciences. – 2010. – Vol.5. – P. 122–134.
Wang J. Mechanical force regulation of myofibroblast differentiation in cardiac fibroblasts // Am. J. Physiol. – 2003. – Vol.285, №5. – H1871–H1881.
Authors retain copyright of their work and grant the journal the right of its first publication under the terms of the Creative Commons Attribution License 4.0 International (CC BY 4.0), that allows others to share the work with an acknowledgement of the work's authorship.
