Analysis of LMNA gene mutations effect on 3-dimensional structure of lamin A and mode of its interaction with emerin and SREBP1
Abstract
Modelling immunoglobulin-like domain structure of mutant forms of lamin revealed noticeable changes of polypeptide backbone geometry of this part of molecule only at mutation R527C. Exposure of charged amino acids and hydrophobicity profile were more pronouncedly changed, accordingly, under mutations G465D and R482L, that was accompanied by significant change of affinity to emerin and SREBP1. Only for mutation R482L the increase of affinity to both protein partners was revealed whereas for other mutant proteins the affinity increased only to SREBP1 and decreased to emerin.
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References
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