Mg2+ ion effect on stability of double-stranded polynucleotide formed by polyriboinosinic and polyribocytidilic chains

  • V.A. Sorokin Institute for Low Temperature Physics and Engineering, NASU
  • V.A. Valeev Institute for Low Temperature Physics and Engineering, NASU
  • E.L. Usenko Institute for Low Temperature Physics and Engineering, NASU
  • V.A. Karachevtsev Institute for Low Temperature Physics and Engineering, NASU
Keywords: polynucleotides, thermal stability, metal ions

Abstract

Mg2+ effect on the thermal stability of the double-stranded polynucleotide Poly I •Poly C (IC) under conditions close to physiological ones (0.1 M Na+, pH 7) was studied by the differential UV spectroscopy method. The initial increase of the ion concentration ([Mg2+]) leads to the rise of the melting temperature Tm and to the decrease of the absorption hyperchromicity value, conditioned by the heating-induced helix-coil transition (hmax). The last effect is supposed to be a result of the Mg2+ interaction with π-electrons of hypoxanthine or cytosine rings (cation-π-interaction). When [Mg2+] reaches its critical value ([Mg2+]cr  ∼ 10-4M), Tm decreases cooperatively and hmax rises up to values close to those observed in the absence of magnesium. It is supposed that at [Mg2+] = [Mg2+]cr IC transits into a new structural state. With [Mg2+]>[Mg2+]cr Tm of the polynucleotide increases again and hmax lowers slightly.

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Author Biographies

V.A. Sorokin, Institute for Low Temperature Physics and Engineering, NASU

47 Lenin Ave., Kharkov, 61103, Ukraine

V.A. Valeev, Institute for Low Temperature Physics and Engineering, NASU

47 Lenin Ave., Kharkov, 61103, Ukraine

E.L. Usenko, Institute for Low Temperature Physics and Engineering, NASU

47 Lenin Ave., Kharkov, 61103, Ukraine

V.A. Karachevtsev, Institute for Low Temperature Physics and Engineering, NASU

47 Lenin Ave., Kharkov, 61103, Ukraine

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Published
2005-06-06
Cited
How to Cite
Sorokin, V., Valeev, V., Usenko, E., & Karachevtsev, V. (2005). Mg2+ ion effect on stability of double-stranded polynucleotide formed by polyriboinosinic and polyribocytidilic chains. Biophysical Bulletin, 1(15), 57-61. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/18096