Nvestigation of thermal destruction of nucleic acids components

  • A.I. Gasan Institute for Radiophysics and Electronics, NASU
  • V.Y. Maleev Institute for Radiophysics and Electronics, NASU
  • A.V. Filipsky Institute for Radiophysics and Electronics, NASU
Keywords: D-ribose, uridin, uridin-5'-tryphosphate, thermal stability, reversibility, microcalorimetry

Abstract

The reversibility of processes that detect at the heating of  D-ribose, uridine, and uridine-5'-triphosphate from 20 to 400°С was studied by the method of differential scanning microcalorimetry. The individual thermograms of samples have been received. It is shown that the processes corresponding endo and exothermic peaks above 200°С are irreversible. The results obtained indicate that the shape of DSC curves at different sealing essentially differs. It is received, that different content of water in a sample affect the displaying processes, namely for humid samples of D-ribose and uridine-5'-triphosphate endothermic peaks above 200°С was absent and the temperature of a maximum of exothermic peaks has decreased. For all samples, the temperatures of the destruction process were determined, and the values of effective heat and effective activation energy were calculated.

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Author Biographies

A.I. Gasan, Institute for Radiophysics and Electronics, NASU

12 Proskura st., Kharkov, 61085, Ukraine

V.Y. Maleev, Institute for Radiophysics and Electronics, NASU

12 Proskura st., Kharkov, 61085, Ukraine

A.V. Filipsky, Institute for Radiophysics and Electronics, NASU

12 Proskura st., Kharkov, 61085, Ukraine

References

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Published
2005-06-06
Cited
How to Cite
Gasan, A., Maleev, V., & Filipsky, A. (2005). Nvestigation of thermal destruction of nucleic acids components. Biophysical Bulletin, 1(15), 11-14. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/17977