The role of metal ions in the fibrillization of lysozyme and insulin in vitro

  • K. O. Vus V.N. Karazin Kharkiv National University
  • V. M. Trusova V.N. Karazin Kharkiv National University
  • G. P. Gorbenko V.N. Karazin Kharkiv National University
  • E I. Slobozhanina Institute of Biophysics and Cell Engineering
  • L. M. Lukyanenko Institute of Biophysics and Cell Engineering
  • R. Sood Aalto University
  • P. Kinnunen Aalto University
Keywords: amyloid fibrils, insulin, lysozyme, metal ions, Thioflavin T

Abstract

The effects of metal ions on the fibrillization of lysozyme and insulin were investigated using a
Thioflavin T fluorescence assay and transmission electron microscopy. The incubation of the proteins in
acidic buffer at high temperature in the presence of substoichiometric concentrations of Сu2+, Zn2+, Fe3+
and Al3+ primarily led to the decrease in lag phase of fibril growth and protein aggregation rate compared to those in control samples. Furthermore, Сu2+ and Fe3+ showed a stronger influence on the protein fibrillization than Zn2+ and Al3+. Specifically, Сu2+ and Fe3+ provoked the formation of insulin amyloid fibrils, having a «fuzzy» appearance, while the amyloid-like insulin aggregates grew in the presence of Zn2+. These results highlight the potential of metal ions as prospective therapeutic agents against human«conformational» diseases.

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Author Biographies

K. O. Vus, V.N. Karazin Kharkiv National University

Svobody Sq., 4, Kharkiv 61022, Ukraine

V. M. Trusova, V.N. Karazin Kharkiv National University

Svobody Sq., 4, Kharkiv 61022, Ukraine

G. P. Gorbenko, V.N. Karazin Kharkiv National University

Svobody Sq., 4, Kharkiv 61022, Ukraine

E I. Slobozhanina, Institute of Biophysics and Cell Engineering

Academicheskaya, 27, Minsk 220072, Belarus

L. M. Lukyanenko, Institute of Biophysics and Cell Engineering

Academicheskaya, 27, Minsk 220072, Belarus

R. Sood, Aalto University

Otakaari, 3, Еspoo FI-00076, Finland

P. Kinnunen, Aalto University

Otakaari, 3, Еspoo FI-00076, Finland

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Cited
How to Cite
Vus, K. O., Trusova, V. M., Gorbenko, G. P., Slobozhanina, E. I., Lukyanenko, L. M., Sood, R., & Kinnunen, P. (1). The role of metal ions in the fibrillization of lysozyme and insulin in vitro. Biophysical Bulletin, 2(30). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/1596

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