Spectral behavior of novel benzanthrone probe in model membranes
Keywords:
benzanthrone dye, liposomes, phosphatidylcholine, cardiolipin, cholesterol, lysozyme
Abstract
The present study was undertaken to evaluate the sensitivity of a newly synthesized benzanthrone dye to
the changes in physicochemical properties of lipid bilayer. It was shown that the dye under study is nonemissive in buffer but exhibites strong fluorescence in lipid phase. Partitioning of AM15 into model
membranes composed of zwitterionic lipid phosphatidylcholine (PC) and its mixtures with anionic lipid
cardiolipin and cholesterol was followed by significant increase of fluorescence quantum yield. Analysis
of the partition coefficients showed that inclusion of cardiolipin and choleterol into phosphatidylcholine
bilayer gives rise to the decrease of AM15 incorporation into lipid phase compared to the neat
phosphatidylcholine membrane. It is assumed that AM15 resides in the hydrophobic bilater region, being
oriented parallel to the lipid acyl chains
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References
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2. Stubbs C.D., Williams B.W. In Lacowicz J.R. (Ed.) Topics in fluorescence spectroscopy // Plenum press, New York. – 1992. – V. 3. – P. 231-271.
3. Epand R.F., Kraayenhof R., Sterk G.J., Sang H., Epand R.M. Fluorescent probes of membrane surface proper-ties // Biochim. Biophys. Acta. – 1996. – V. 1284. – P. 191-195.
4. Lentz B.R. Use of fluorescent probes to monitor molecular order and motion within liposome bilayers // Chem. Phys. Lipids. – 1993. – V. 64. – P. 99-116.
5. Smith J.C. Potential-sensitive molecular probes in membranes of bioenergetic relevance // Biochim. Biophys. Acta. – 1990. – V. 1016. – P. 1-28.
6. Haugland R.P. Handbook of fluorescent probes and research chemicals // Molecular Probes, Leiden. 2001.
7. Visser N.V., A. van Hoek, Visser A.J.W.J.G., Frank J., Apell H.J. Clarke R.G. time-resolved fluorescence investigations of the interaction of the voltage-sensitive probe RH421 with lipid membranes and proteins // Bio-chemistry. – 1995. – V. 34. – P. 11777-11784.
8. Krishna M.M.G., Periasamy N. Fluorescence of organic dyes in lipid membranes: site of solubilization and effects of viscosity and refractive index on lifetimes // J. Fluoresc. – 1998. – V. 8. – P. 81-91.
9. Mui B., L. Chow L., Hope M.J. Extrusion technique to generate liposomes of defined size // Meth. Enzymol. – 2003. – V. 367. – P. 3 - 14.
10. Bartlett G. Phosphorus assay in column chromatography // J. Biol. Chem. – 1959. – V. 234. – P. 466 - 468.
11. Valeur B. Molecular Fluorescence:Principles and Applications // Wiley-VCH Verlag GmbH. 2001.
12. Lakowicz J.R. Principles of fluorescent spectroscopy// Springer: Singapore. 2006.
13. Santos N.C., Prieto M., Castanho M.A.R.B. Quantifying molecular partition into model systems of biomem-branes: an emphasis on optical spectroscopic methods // Biochim. Biophys. Acta. – 2003. – V. 1612. – P. 123 - 135.
14. Ivkov V.G., Berestovsky G.N. Dynamic Structure of Lipid Bilayer// Nauka, Moscow. 1981.
15. Stewart L.C., Kates M. and Smith I.C.P. Synthesis and characterization of deoxy analogues of diphytanyl-glycerol phospholipids // Chem. Phys. Lipids. – 1988. – V. 48. – P. 177-188.
16. Hubner W., Mantsch H.M. and Kates M. Intramolecular hydrogen bonding in cardiolipin // Biochim. Bio-phys. Acta. – 1991. – V. 1066. – P. 166-174.
17. Shibata A., Ikawa K., Shimooka T., Terada H. Significant stabilization of the phosphatidylcholine bilayer structure by incorporation of small amounts of cardiolipin // Biochim. Biophys. Acta. – 1994. – V. 1192. – P. 71-78.
18. Yeagle. P., Hutton W., Huang C. and Martin R. Phospholipid head-group conformations; intermolecular interactions and cholesterol effects. // Biochemistry. – 1977. – V. 16. – P. 4344–4449.
19. Pasenkiewicz-Gierula M., Rog T., Kitamura K., and Kusumi A. Cholesterol effects on the phosphatidylcho-line bilayer polar region: a molecular simulation study.// Biophys. J. – 2000. – V. 78. – P. 1376–1389.
20. Ho C., Slater S., and Stubbs C. Hydration and order in lipid bilayers. // Biochemistry. – 1995. – V. 34. – P. 6188–6195.
21. Jendrasiak G. L. and Hasty J. The hydration of phospholipids. // Biochim. Biophys. Acta. – 1974. – V. 337. – P. 79–91.
22. Merkle H., Subczynski W. K., and Kusumi A. Dynamic fluorescence quenching studies on lipid mobilities in phosphotidylcholine-cholesterol membranes. // Biochim. Biophys. Acta. – 1987. – V. 897. – P. 238–348.
23. Bittman R. and Blau L. The phospholipid-cholesterol interaction. Kinetics of water permeability in lipo-somes. // Biochemistry. – 1972. – V. 11. – P. 4831–4839.
24. Bach D. and Miller I. R. Hydration of phospholipid bilayers in the presence and absence of cholesterol. // Biochim. Biophys. Acta. – 1998. – V. 1368 – P. 216–224.
25. Straume M. and Litman B. Influence of cholesterol on equilibrium and dynamic bilayer structure of unsatu-rated acyl chain phosphatidylcholine vesicles as determined from higher order analysis of fluorescence anisot-ropy decay. // Biochemistry. – 1987. – V. 26. – P. 5121–5126.
26. Rog T. and Pasenkiewicz-Gierula M. Cholesterol effects on the phosphatidylcholine bilayer nonpolar region: a molecular simulation study. // Biophys. J. – 2001. – V. 81. – P. 2190–2202.
27. Demel R. A. and B. de Kruijff The function of sterols in membranes. // Biochim. Biophys. Acta. – 1976. – V. 457. – P. 109–132.
28. Kinnunen PKJ, Koiv A, Lehtonen JYA, Rytomaa M, Mustonen P. Lipid dynamics and peripheral interac-tions of proteins with membrane surface. // Chem. Phys. Lipids. – 1991. – V. 73. – P. 181–207.
29. Ge M., Freed J.H., Hydration, structure, and molecular interactions in the headgroup region of dioleoylphos-phatidylcholine bilayers: an electron spin resonance study. // Biophys. J. – 2003. – V. 85. – P. 4023–4040.
30. Vedamuthu M, Singh S, Onganer Y, Bessire DR, Yin M, Quitevis EL, Robinson GW Universality in isom-erization reactions in polar solvents. // J. Phys. Chem. – 1996. – V. 100. – P. 11907–11913
Cited
How to Cite
Zhytniakivska, O. A., Kutsenko, O. K., Trusova, V. M., Gorbenko, G. P., Kirilova, E. M., Kirilov, G. K., & Kalnina, I. (1). Spectral behavior of novel benzanthrone probe in model membranes. Biophysical Bulletin, 1(26). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2714
Section
Methods of biophysical investigations
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