TY - JOUR AU - Vus, K. O. AU - Trusova, V. M. AU - Gorbenko, G. P. AU - Slobozhanina, E I. AU - Lukyanenko, L. M. AU - Sood, R. AU - Kinnunen, P. PY - 1970/01/01 Y2 - 2024/03/28 TI - The role of metal ions in the fibrillization of lysozyme and insulin in vitro JF - Biophysical Bulletin JA - Bioph. Bull. VL - 2 IS - 30 SE - Molecular biophysics DO - UR - https://periodicals.karazin.ua/biophysvisnyk/article/view/1596 SP - AB - The effects of metal ions on the fibrillization of lysozyme and insulin were investigated using aThioflavin T fluorescence assay and transmission electron microscopy. The incubation of the proteins inacidic buffer at high temperature in the presence of substoichiometric concentrations of Сu2+, Zn2+, Fe3+and Al3+ primarily led to the decrease in lag phase of fibril growth and protein aggregation rate compared to those in control samples. Furthermore, Сu2+ and Fe3+ showed a stronger influence on the protein fibrillization than Zn2+ and Al3+. Specifically, Сu2+ and Fe3+ provoked the formation of insulin amyloid fibrils, having a «fuzzy» appearance, while the amyloid-like insulin aggregates grew in the presence of Zn2+. These results highlight the potential of metal ions as prospective therapeutic agents against human«conformational» diseases. ER -