Detection of Lysozyme Amyloid Fibrils Using Trimethine Cyanine Dyes: Spectroscopic and Molecular Docking Studies

  • Olga Zhytniakivska Department of Medical Physics and Biomedical Nanotechnologies, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-2068-5823
  • Uliana Tarabara Department of Medical Physics and Biomedical Nanotechnologies, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-7677-0779
  • Atanas Kurutos Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria; Department of Pharmaceutical and Applied Organic Chemistry, Faculty of Chemistry and Pharmacy, Sofia University St. Kliment Ohridski, Sofia, Bulgaria; https://orcid.org/0000-0002-6847-198X
  • Kateryna Vus Кафедра медичної фізики та біомедичних нанотехнологій, Харківський національний університет імені В.Н. Каразіна, Харків, Україна https://orcid.org/0000-0003-4738-4016
  • Valeriya Trusova Department of Medical Physics and Biomedical Nanotechnologies, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-7087-071X
  • Galyna Gorbenko Department of Medical Physics and Biomedical Nanotechnologies, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-0954-5053
Keywords: Trimethine cyanine dyes, lysozyme, amyloid fibrils

Abstract

Due to their unique photophysical and photochemical properties and high sensitivity to the beta-pleated motifs, cyanine dyes have found numerical applications as molecular probes for the identification and characterization of amyloid fibrils in vitro and the visualization of amyloid inclusions in vivo. In the present study the spectroscopic and molecular docking techniques have been employed to evaluate the amyloid sensitivity and the mode of interaction between the trimethine cyanine dyes and native (LzN) and fibrillar (LzF) lysozyme. It was found that the trimethine association with non-fibrilar and fibrillar lysozyme is accompanied by the changes in dye aggregation extent. The molecular docking studies between trimethine dyes and lysozyme in the native and amyloid states indicate that: i) trimethines tend to form the most stable complexes with deep cleft of the native lysozyme; ii) the dye binding with non-fibrillar protein is governed by the hydrophobic interactions, π-stacking contacts between aromatic or cyclopentane ring of the cyanine and Trp in position 63 or 108 and hydrogen bonds between the OH-groups of the trimethines and acceptor atoms of Asp 101 (AK3-7) and Gln 57 (AK3-8) of LzN; iii) cyanine dyes form the energetically most favorable complexes with the groove Gly 2-Leu 4/Ser 8-Trp 10 of the lysozyme fibril core; iv) cyanines-LzF interaction is stabilised by hydrobhobic contacts, π-stacking interaction and hydrogen bonds. The dyes AK3-7, AK3-5 and AK3-11 were selected as the most prospective amyloid probes.

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References

P. Pronkin, A. Tatikolov, Molecules, 27(19), 6367 (2022). https://doi.org/10.3390/molecules27196367.

M. Bokan, G. Gellerman, L. Patsenker. Dyes Pigm., 171, 107703 (2019). https://doi.org/10.1016/j.dyepig.2019.107703.

M. Guo, P. Diao, Y.-J. Ren, F. Meng, H. Tian and S.-M. Cai, Sol. Energy Mater. Sol. Cells, 88, 33–35 (2005). https://doi.org/10.1016/j.solmat.2004.10.003.

C. Shi, J.B. Wu, D. Pan. J. Biomed. Opt. 21(5), 05901 (2022). https://doi.org/10.1117/1.JBO.21.5.050901.

G. D. Pelle, A. D. Lopez, M. S. Fiol, Int. J. Mol. Sci., 22(13), 6914, (2021). https://doi.org/10.3390/ijms22136914.

O. Cavuslar, and H. Unal, RSC Advances. 5, 22380-22389 (2015). https://doi.org/10.1039/C5RA00236B

H. L. Yang, S.Q. Fang, Y.W. Tang, et al., Eur. J. Med. Chem. 179, 736-73 (2019). http://dx.doi.org/10.1016/j.ejmech.2019.07.005.

X. Mu, F. Wu, R. Wang, Z. Huang, et al., Sens Actuators B. Chemical., 338, 29842, (2021). https://doi.org/10.1016/j.snb.2021.129842.

K.D. Volkova, V.B. Kovalska, O.A. Balanda, R.J. Vermeij, V. Subramaniam, Y.L. Slominskii, S.M. Yarmoluk, J. Biochem. Biophys. Methods, 70, 727–733, (2007). https://doi.org/10.1016/j.jbbm.2007.03.008.

V.B. Kovalska, M.Yu. Losytskyy, O.I. Tolmachev, et al., J. Fluoresc. 22, 1441–1448, (2012). https://doi.org/10.1007/s10895-012-1081-x.

J. Yan, J. Zhu, K. Zhou, et al., Chem. Commun. 53, 1441–1448, (2017). https://doi.org/10.1039/C7CC05056A.

T. Smidlehner, H. Bonnet, S. Chierici, I. Piantanida, Bioorg. Chem.,104, 104196, (2020). https://doi.org/10.1016/j.bioorg.2020.104196.

R. Sabate, J. Estelrich, Biopolymers, 72(6), 455-463, (2003).https://doi.org/10.1002/bip.10485.

G.Q. Gao, A.W. Xu, RSC Adv., 3, 21092-21098, (2013). https://doi.org/10.1039/C3RA43259A.

O. Zhytniakivska, A. Kurutos, U. Tarabara, K. Vus, V. Trusova, G. Gorbenko, N. Gadjev. And T. Deligeorgiev, J. Mol. Liq. 311, 113287 (2020), https://doi.org/10.1016/j.molliq.2020.113287

S. Chernii, Y. Gerasymchuk, M. Losytskyy, D. Szymanski, et al., PLOS One, 16(1), e0243904. (2021). https://doi.org/10.1371/journal.pone.0243904.

K. Vus, U. Tarabara, A. Kurutos, O Ryzhova, G. Gorbenko, et al., Mol Biosyst, 13(5), 1970-1980, (2017). https://doi.org/10.1039/c7mb00185a

H.L. Yang, S.Q. Fang, Y.W. Tang, et al. Eur. J. Med. Chem, 179, 736-743, (2019). https://doi.org/10.1016/j.ejmech.2019.07.005.

K. Vus, M. Girych, V. Trusova, et al. J Mol Liq, 276, 541-552 (2019). https://doi.org/10.1016/j.molliq.2018.11.149.

M. Bacalum, B. Zorila, M. Radu, Anal. Biochem., 440, 123-129, (2013). https:// doi.org/10.1016/j.ab.2013.05.031.

S. Dallakyan, and A.J. Olson, Methods Mol. Biol. 1263, 243-250 (2015), https://doi.org/10.1007/978-1-4939-2269-7_19.

S. Salentin, S. Schreiber, V.Joachim Haupt, M.F. Adasme, and M. Schroeder, Nucleic Acids Res. 43, W443-W447 (2015), https://doi.org/10.1093/nar/gkv315.

P. Csizmadia, In: Proceedings of ECSOC-3, the third international electronic conference on synthetic organic chemistry, 367-369 (1999), https://doi.org/10.3390/ECSOC-3-01775

M.D. Hanwell, D.E. Curtis, D.C. Lonie, T. Vandermeersch, E. Zurek, and G.R. Hutchison, J. Cheminform. 4, 17 (2012), https://doi.org/10.1186/1758-2946-4-17.

V. Trusova, East Eur. J. Phys. 2, 51-58 (2015), https://doi.org/10.26565/2312-4334-2015-2-06.

P.L. Donabedian, M. Evanoff, F.A. Monge, D.G. Whitten, and E.Y. Chi, ACS Omega, 2, 3192-3200 (2017), https://doi.org/10.1021/acsomega.7b00231

Published
2022-12-06
Cited
How to Cite
Zhytniakivska, O., Tarabara, U., Kurutos, A., Vus, K., Trusova, V., & Gorbenko, G. (2022). Detection of Lysozyme Amyloid Fibrils Using Trimethine Cyanine Dyes: Spectroscopic and Molecular Docking Studies. East European Journal of Physics, (4), 213-221. https://doi.org/10.26565/2312-4334-2022-4-22

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