Studying the Dependence of the Toxic Effect on the Time of the Toxicant's Contact with the Algae Culture
Abstract
Relevance. The intensity of photosynthesis is the most common toxicity test when using algae as test objects. All methods for determining photosynthesis are based on measuring the rate of oxygen evolution or absorption of carbon dioxide in an incubation medium before and after a certain exposure of algae culture to light.
Purpose. Determination of threshold concentrations of toxic substances for this method and study of the dependence of the type of dose-value of the toxic effect.
Results. Toxicity bioassay by evaluating the photosynthetic activity of algae is possible only for wastewater with acute toxicity. A direct dependency between the magnitude of the toxic effect and the duration
of contact of algae with toxicants is observed for about an hour. A further increase in the contact time almost does not increase the toxic effect.
Conclusions. By increasing the contact time of algae with toxicants, it is possible to significantly increase the sensitivity of the method and, possibly, use it to evaluate low-toxic wastewater. However, for the final conclusions about the limits of sensitivity of the method, and, therefore, about the scope of its application, additional studies are needed.
Methods. Biotesting with the help of microalgae
Downloads
References
Filenko, O. F. (1988). Aquatic toxicology. Chernogolovka: Institute of Problems of Chemical Physics, USSR Academy of Sciences, 155. (In Russian).
Patin, S. A. (1991). Ecological and toxicological aspects of the study and quality control of the aquatic envi-ronment. Hydrobiological journal, 2 (6), 71-75. (In Russian).
Patin, S. A. (2004). Ecological and toxicological approaches to assessing the impact on the marine environ-ment and biological resources. Actual problems of aquatic toxicology, Borok: Rybinsk Printing House, 34-60. (In Russian).
Krajnyukov, A. N., Krajnyukova, A. N., Chistyakova, E. O. (2012). Monitoring the return water of a chemical plant and the quality of water in the control sections of a water body. Problems of environmental protection and ecological safety, 35, 51-60. (In Russian).
Krainiukov, O. M. (2013). Bioecological methods for the study of aquatic landscapes. Physical Geography and Geomorphology, 3 (71), 158-167. (In Ukrainian).
Krainiukov, O. M., Kryvytska, I. A. (2016). Establishment of ecological safety standards for fishery water management based on landscape ecological approach (on the example of morpholine). Young scientist, 12, 15-18. (In Ukrainian).
Nikookar, K., Moradshahi, A., Hosseini, L. (2005). Physiological responses of Dunaliella sa-lina and Dunaliella tertiolecta to copper toxicity. Biomol. Eng., 22, 141-146. https://doi.org/10.1016/j.bioeng.2005.07.001
Beneche, G. (1977). Automatisierung der auswertur einer al-genheteste hemmung der kreichenbewegung einer blaualge (Phornidium sp.) durch Digit. Z. Waasser und Abwasser-Forsch, (6), 195-197.
Raso, J., Rachlin, I. (1977). The effect of cadmium, copper, mercuru, zincum and lead on cell division growth and chlorophyll a content a content of the chlorophyte Chl. vulgaris. Bull. Torreu. Bot. Club., (3), 226-233.
Garcíaríos, V, Freilepelegrín, Y, Robledo, D, Mendoza-cózatl D, Moreno-sánchez R, Gold-bouchot G. (2007). Cell wall composition affects Cd2+ accumulation and intracellular thiol peptides in marine red algae. Aquatic Toxicol, 81, 65–72. https://doi.org/10.1016/j.aquatox.2006.11.001
Braginskij, L. P. (2004). Some results of studies on aquatic toxicology in Ukraine. Actual problems of aquatic toxicology. Borok, 11-34. (In Russian).
Cullimore, D. R. (1966). A qualitative method of assessing the available nitrogen, potassium and phosphorus in the soil. J. Sci. Food Agric., 17, 321-323. Available at: https://doi.org/10.1002/jsfa.2740170709
Radix, P., Leonard, M., Papantoniou, C. et al (2000). Comparison of four chronic toxicity tests using algae, bacteria, and invertebrates assessed with sixteen chemicals. Ecotoxicol Environ Saf, 47, 186–194.
María Elena Sáenz, Walter Darío Di Marzio and Jose Luis Alberdi (2012). Assessment of Cyfluthrin commercial formulation on growth, photosynthesis and catalase activity of green algae, Pesticide Biochemistry and Physiology, 104 (1), 50-57. https://doi.org/10.1016/j.pestbp.2012.07.001
Heijerick, D. G., Bossuyt, B. T. A., De Schamphelaere, K. A. C., Indeherberg, M., Mingazzini, M. and Janssen, C. R. (2005). Effect of Varying Physicochemistry of European Surface Waters on the Copper Toxicity to the Green Alga Pseudokirchneriella subcapitata. Ecotoxicology, 14 (6), 661-670. https://doi.org/10.1007/s10646-005-0014-8
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication of this work under the terms of a license Creative Commons Attribution License 4.0 International (CC BY 4.0).
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
