Encapsulation of europium chelates into lipid vesicles

  • E. V. E.V. Pakhomova V.N. Karazin Kharkov National University
  • O. K. Kutsenko V.N. Karazin Kharkov National University
  • G. P. Gorbenko V.N. Karazin Kharkov National University
  • V. M. Trusova V.N. Karazin Kharkov National University
  • T. Deligeorgiev Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia
  • A. Vasilev Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia
  • S. Kaloianova Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia
  • N. Lesev Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia
Keywords: europium chelates, doxorubicin, liposomes, fluorescence quenching

Abstract

The partitioning of europium chelates (EC) between the aqueous and lipid phases was studied. The
possibility of coencapsulation of two anticancer drugs (doxorubicin and europium complex) into lipid
vesicles was evaluated. Partition coefficients obtained by equilibrium dialysis technique indicate that EC
can be effectively entrapped by the lipid phase of liposomes. Measurement of intrinsic fluorescence of
doxorubicin suggests that this agent is located mainly in the inner aqueous phase of liposomes. EC were
found to quench the fluorescence of Laurdan and pyrene. The effect of doxorubicin on incorporation of
europium complexes into lipid bilayer was demonstrated to depend on chemical structure of chelates.

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Author Biographies

E. V. E.V. Pakhomova, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

O. K. Kutsenko, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

G. P. Gorbenko, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

V. M. Trusova, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

T. Deligeorgiev, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

Bulgaria

A. Vasilev, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

Bulgaria

S. Kaloianova, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

Bulgaria

N. Lesev, Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia

Bulgaria

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How to Cite
E.V. Pakhomova, E. V., Kutsenko, O. K., Gorbenko, G. P., Trusova, V. M., Deligeorgiev, T., Vasilev, A., Kaloianova, S., & Lesev, N. (1). Encapsulation of europium chelates into lipid vesicles. Biophysical Bulletin, 1(26). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2707

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