Spectral behavior of novel benzanthrone probe in model membranes

  • O. A. Zhytniakivska V.N. Karazin Kharkiv National University
  • O. K. Kutsenko V.N. Karazin Kharkiv National University
  • V. M. Trusova V.N. Karazin Kharkiv National University
  • G. P. Gorbenko V.N. Karazin Kharkiv National University
  • E. M. Kirilova Daugavpils University
  • G. K. Kirilov Daugavpils University
  • I. Kalnina Latvia
Keywords: benzanthrone dye, liposomes, phosphatidylcholine, cardiolipin, cholesterol, lysozyme

Abstract

The present study was undertaken to evaluate the sensitivity of a newly synthesized benzanthrone dye to
the changes in physicochemical properties of lipid bilayer. It was shown that the dye under study is nonemissive in buffer but exhibites strong fluorescence in lipid phase. Partitioning of AM15 into model
membranes composed of zwitterionic lipid phosphatidylcholine (PC) and its mixtures with anionic lipid
cardiolipin and cholesterol was followed by significant increase of fluorescence quantum yield. Analysis
of the partition coefficients showed that inclusion of cardiolipin and choleterol into phosphatidylcholine
bilayer gives rise to the decrease of AM15 incorporation into lipid phase compared to the neat
phosphatidylcholine membrane. It is assumed that AM15 resides in the hydrophobic bilater region, being
oriented parallel to the lipid acyl chains

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Author Biographies

O. A. Zhytniakivska, V.N. Karazin Kharkiv National University

4 Svobody Sq., Kharkiv, 61077, Ukraine

O. K. Kutsenko, V.N. Karazin Kharkiv National University

4 Svobody Sq., Kharkiv, 61077, Ukraine

V. M. Trusova, V.N. Karazin Kharkiv National University

4 Svobody Sq., Kharkiv, 61077, Ukraine

G. P. Gorbenko, V.N. Karazin Kharkiv National University

4 Svobody Sq., Kharkiv, 61077, Ukraine

E. M. Kirilova, Daugavpils University

Latvia

G. K. Kirilov, Daugavpils University

Latvia

I. Kalnina, Latvia

Latvia

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Cited
How to Cite
Zhytniakivska, O. A., Kutsenko, O. K., Trusova, V. M., Gorbenko, G. P., Kirilova, E. M., Kirilov, G. K., & Kalnina, I. (1). Spectral behavior of novel benzanthrone probe in model membranes. Biophysical Bulletin, 1(26). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2714
Section
Methods of biophysical investigations

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