INFLUENCE OF γ-IRRADIATION ON STATE OF DNA ION-HYDRATION SHELL

  • V. А. Kashpur Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine
  • О. V. Dubovitskaya Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine
  • А. А. Krasnitskaya Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine
  • V. Ya. Maleev Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine
Keywords: DNA, electrophoresis

Abstract

The effect of γ-radiation on the state of DNA hydration shell has been investigated. The changes of dielectric permittivity of DNA aqueous solutions irradiated at doses 19, 370, and 1650 Gy have been studied using the differential dielectrometric method. The DNA state was checked by conductance-measuring and electrophoresis methods. Some noticeable changes of DNA hydration at 370 and 1650 Gy have been found. It has been shown that the effects revealed are due to the breach of water backbone structure arising from double helix reconstructions which appear to be a result of radiation deoxyribosyl lesions and also to the appearance of DNA radiation-altered bases.

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Author Biographies

V. А. Kashpur, Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine

12 Acad. Proskura str., Kharkov 310085, Ukraine

О. V. Dubovitskaya, Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine

 12 Acad. Proskura str., Kharkov 310085, Ukraine

А. А. Krasnitskaya, Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine

 12 Acad. Proskura str., Kharkov 310085, Ukraine

V. Ya. Maleev, Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine

12 Acad. Proskura str., Kharkov 310085, Ukraine

References

Рябченко Н.И. Радиация и ДНК. М. Атомиздат, 1979. 192с.

Шарпатый В.А. // Радиобиология. 1992. Т.32. С.180-193.


Зенгер В. Принципы структурной организации нуклеиновых кислот. М. Мир, 1987. 584с. .


Swarts S.G., Sevilla M.D., Becker D., Tokar C.J.. Wheeler K.T. // Radiat. Res. 1992. V.129. P.333-344.


Pankowski E. // Studia biophys. 1988. V.128. P.27-36.


Остерман Л.А. Методы исследования белков и нуклеиновых кислот. М. Наука, 1981. 286с.


Кашпур В.А., Малеев В.Я., Щеголева Т.Ю. // Мол. биол. 1976. Т. 10. С.568-575.


Maleev V.Ya., Kashpur V.A., Glibitsky G.M., Krasnitskaya A.A., Veretelnik Ye.V. // Biopolymers. 1987. V.26. P. 1965-1970.


Adinarayana M., Bothe E., Schulte-Frohlinde D. // Int. J. Radiat. Biol. 1988. V.54. P.723-733.

Umehara T, Kuwabara S., Mashimo S., Yagihara S. // Biopolymers. 1990. V.30. P.649-656.

Trumbore C. N., Myers Y. N., Hyde C.K., Hudson R.D., Rhodes C.N., Masselink J.K. // Int. J. Radiat. Biol. 1994. V. 66. P.479-483.


Collyns В., Okada S., Scholes G, Weiss J.J., Wheeler C.M. // Radiat. Res. 1965. V. 25. P.526-536.


Fuciarelli A.F., Wegher B.J., Blakely W.F., Dizdaroglu M. // Int. J. Radiat. Biol. 1990. V.58. P.397-415.

Dizdaroglu M. // Int. J. Radiat. Biol. 1992. V. 62. P. 175-183.


Henle E.S., Roots R, Holley W.R., Chatterjee A. // Radiat. Res. 1995. V. 143. P. 144-150.
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How to Cite
KashpurV. А., DubovitskayaО. V., KrasnitskayaА. А., & Maleev, V. Y. (1). INFLUENCE OF γ-IRRADIATION ON STATE OF DNA ION-HYDRATION SHELL. Biophysical Bulletin, 1(1). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2059
Section
Action of physical agents on biological objects