Theoretical analysis of the efficiency of linear and two-step freezing regimens for mammalian embryo cryopreservation

  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of NASU
Keywords: cryopreservation, cooling rate, rapid two-step freezing, mammalian embryo

Abstract

The research was aimed at the theoretical evaluation of the application expediency of non-linear two-step freezing regimens for mammalian embryo cryopreservation. Basing on the comparison of theoretical dependency of intracellular solution overcooling on time (or temperature) under linear and two-step freezing regimens there the advantages of the latter for mammalian embryo cryopreservation were established and explained. Theoretical evaluation of optimal cooling rate by the two-factor cryodamage theory for two-cell murine embryos coincides with experimental data

Downloads

Download data is not yet available.

Author Biography

M. V. Ostankov, Institute for Problems of Cryobiology and Cryomedicine of NASU

23 Pereyaslavskaya str., Kharkov, Ukraine 61015

References

1. Ланда П.С. Автоколебания в системах с конечным числом степеней свободы.-М. :Наука. Гл. ред физ.-мат. лит-ры.-1980.-360с.

2. Е. А. Гордиенко, Н.С. Пушкарь. Физические основы низкотемпературного консервирования клеточных суспензий. Киев: Наук, думка, 1994.- 143с.

3. Mazur P. Cryobiology: the freezing of biological objecls//Science.-1970.-168. N3934/-p.939-949.

4. Котык А., Яначек К. Мембранный транспорт -М;Мир.-1980.-341с.

5. Wood M.J., Farrant J. Preservation mouse embryos by two-step frrezing//Cryobiology.-1980.-17-p. 178-180.
Published
2008-09-29
Cited
How to Cite
Ostankov, M. V. (2008). Theoretical analysis of the efficiency of linear and two-step freezing regimens for mammalian embryo cryopreservation. Biophysical Bulletin, 1(20), 73-78. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/1583
Section
Biophysics of complex systems