TY - JOUR AU - Ostankov, M. V. PY - 2008/09/29 Y2 - 2024/03/28 TI - Theoretical analysis of the efficiency of linear and two-step freezing regimens for mammalian embryo cryopreservation JF - Biophysical Bulletin JA - Bioph. Bull. VL - 1 IS - 20 SE - Biophysics of complex systems DO - UR - https://periodicals.karazin.ua/biophysvisnyk/article/view/1583 SP - 73-78 AB - The research was aimed at the theoretical evaluation of the application expediency of non-linear two-step freezing regimens for mammalian embryo cryopreservation. Basing on the comparison of theoretical dependency of intracellular solution overcooling on time (or temperature) under linear and two-step freezing regimens there the advantages of the latter for mammalian embryo cryopreservation were established and explained. Theoretical evaluation of optimal cooling rate by the two-factor cryodamage theory for two-cell murine embryos coincides with experimental data ER -