IR-SPECTROSCOPIC STUDY OF Cu2+ ION INTERACTION WITH DNA IN AQUEOUS SOLUTIONS CONTAINING ADDITIONS OF 1,2-PROPANEDIOL AND GLYCEROL

  • Е. V. Hackl Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine
  • S. V. Kornilova Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine
  • Yu. P. Blagoi Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine
Keywords: DNA, IR-spectroscopy, glycerin

Abstract

The present work studied the Cu2+ ion interaction with DNA in aqueous solutions containing low (up to 20 v %) additions of glycerol or 1,2-propanediol at 29 and 45°C by IR-spectroscopy. Cu2+ ions were shown to induce the DNA transition into compact form in aqueous and water-glycerol (propanediol) solutions both at 29 and at 45°C. This process is of high positive cooperativity. 1,2-propanediol added to the DNA aqueous solution increases cooperativity of the DNA transition into compact state and decreases the Cu2+ ions concentration required to induce DNA compactisation. The 1,2-propanediol effect increases moriotonously with the rise of its volume concentration (in the concentration range 0-20 v %) at 29 and 45°C. In contrast to propanediol, the glycerol effect on DNA compactisation is of non-monotonous character: small (4 v %) glycerol additions to DNA aqueous solution decrease cooperativity of the DNA transition into compact state at 29°C. Comparison of effects of small additions of 1,2-propanediol and glycerol for which dielectric permeability of solution changes practically the same, on the Cu2+-induced DNA condensation, permits the conclusion that the DNA condensation induced by the Cu2+ ions depends not only on dielectric permeability but on the structure of the solution formed.

Downloads

Download data is not yet available.

Author Biographies

Е. V. Hackl, Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine

47 Lenin Ave., Kharkov 310164, Ukraine

S. V. Kornilova, Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine

47 Lenin Ave., Kharkov 310164, Ukraine

Yu. P. Blagoi, Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine

 47 Lenin Ave., Kharkov 310164, Ukraine

References

1. Hackl Е., Kornilova S., Kapinos L.et.al. // J. Mol. Struct. 1997. V. 408/409. P.229-232.


2. Murphy L.D., Zimmerman S.B. // Biophys Chem. 1995. V. 57 (1). P. 71-92.


3. Веселков АН. Влияние внешних факторов на конформацию молекулы нуклеиновой кислоты в растворе. Дис..д-р физ.-мат. наук.Севастополь. 1988. 463с.


4. Ахрем A.A., Егорова В.П., Егоров A.C. и др. // Биополимеры и клетка 1989. вып.5. N5. С. 44-48.


5. Благой Ю.П., Галкин В.Л.. Гладченко Г.О.и др. Металлокомплексы нуклеиновых кислот в растворах. Киев: Наукова думка. 1991. - 272 с.


6. Fischman Е. // Appl. Opt. 1962. N. 1. 493.


7. Малеев В.Я., Семенов М.А. //Биофизика 1971. Т. 16. вып. 3. С. 389-397.


8. Кальвин H.H., Вельяминов С. Ю. // ЖПС. 1987. N 4. С.592-597.


9. Бабушкин A. A., Бажулин П. А., Королев Ф. В. и др. Методы спектрального анализа. М., МГУ. 1962. 273 с.


10. Taillandier Е., Liquier J. // Methods in enzymol. 1990. V. 211. P. 307-335.


11. Ahmad R., Naoui М., Neault J.F. et.al. // J. Biomol. Struct. Dyn. 1996. 13(5). P. 795-802.


12. Kornilova S.. Hackl E., Kapinos L., et.al. // Acta Biochim. Polon.1998. V. 45. N 1. P. 107-117.


13. Arscott P.. Ma Ch., Wenner J., Bloomfield V.// Biopolymers 1995. V. 36. P. 345-364.


14. Pelta J., Livolant F., Sikorav J.-L. // J of Biological Chem. V. 271. N. 10. P. 5656-5662.


15. Bloomfield V. //Curr. Struct. Biol. 1996. V.6. P. 334-341.


16. Ma C., Bloomfield V.//Biophys. J. 1994. V.67. P. 1678-1681.


17. Benbasat J.A.// Biochemistry 1984. V. 23. P. 3609-3619.


18. Хакл E.B.. Корнилова СВ., Благой Ю.П. // Вестник проблем биологии и медицины 1998. N 8. С. 41-51.


19. Tajmir-Riahi Н.А., Ahmad R, Naoui M. // J Biomol Struct Dyn. 1993. V. 10 (5). P. 865-877.


20. Zundel G. Proton polarizability of hydrogen bonds. Series of lectures, Zalzburg. 1997. 250 p.


21. Maniatis Т., Venable J. Jr, Lerman L. // J Mol Biol. 1974. V. 84(1). P. 37-64.

22. Shibata J., Schurr J.//Biopolymers. 1981. V.20. P. 525-549.


23. Duguid J.G., Bloomfield V.A.//Biophys. J. 1995. V. 69. P. 2642-2648.


24. Семенов M.A. Гидратация и структурные переходы нуклеиновых кислот в конденсированном состоянии. Дис... доктора физ.-мат. наук. Харьков. 1988. 416 с.


25. Sorokin V., Blagoi Yu., Valeev V., et.al. // J. Inorg. Biochem.1987, V. 30(2). P. 87-99.


26. Manning G. // Q Rev. Biophys. 1978, V. 11(2). P. 179-246.


27. Wilson R.W., Bloomfield V.A.// Biochemistry 1979. V. 18(11). P. 2192-2196.


28. Хакл E.B., Корнилова С. В., Соловьева А. С. и др. // Проблемы криобиологии 1998., в печати.
Cited
How to Cite
HacklЕ. V., Kornilova, S. V., & Blagoi, Y. P. (1). IR-SPECTROSCOPIC STUDY OF Cu2+ ION INTERACTION WITH DNA IN AQUEOUS SOLUTIONS CONTAINING ADDITIONS OF 1,2-PROPANEDIOL AND GLYCEROL. Biophysical Bulletin, 1(1). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/1928