Study of the influence of ozone on biological systems by methods of optical spectroscopy
Abstract
The spectral characteristics of Saccharomyces cerevisiae yeast cells, model membrane systems (microsomes), and some biopolymers (bovine serum albumin (SAB) and cholinesterase (CE)) before and after the effect of ozone have been studied. The methods of spectrofluorimetry and spectrophotometry have been used. It was shown, that the influence of ozone on studied objects has a precise dose-dependent character. The threshold value of ozone doze at which the destructive action of ozone on yeast cells starts to appear has been established. A decrease of microviscosity of annular lipids under the action of ozone was found. It was shown, that own fluorescence of CE and SAB increases at low doses of ozone. The intensity of own fluorescence of CE and SAB decreases down to the complete disappearance at high doses of ozone. It specifies the severe destruction of these molecules under the action of ozone.
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References
Dehlinger PJ, Schimke RT. Size distribution of membrane proteins of rat liver and their relative rates of degradation. J. Biol. Chem. 1971;246(8):2574-83.
Lowry O, Rosebrough N, Farr A, Randall R. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951;1:265-75.
Morozova TF, Romodanova EA, Diubko TS, Timchenko NN. Spektroskopicheskoe issledovanie vliianiia zamorazhivaniia na plazmu kordzhovoi krovi. Biofizicheskii vestnik. 2002;2(11):110-5. (in Russian)
Vladimirov IuA, Dobretcov GE. Fluorestcentnye zondy v issledovanii biologicheskikh membran. M.:Nauka; 1980. 320p.(in Russian)
Dobretcov GE, Spirin MM, Kariakin AV. Issledovanie s pomoshchiu induktivno-rezonansnogo perenova energii prostranstvennykh vzaimodeistvii mezhdu belkami i lipidami v membranakh mikrosom pecheni. Biokhimiia. 1981;46(3):504-11. (in Russian)
Matus VK, Martynova MA, Skorinko MA. Biologicheskie mebrany. 1999;16:50-6. (in Russian)
Volotovkii ID, editor. Biofizicka zhifykh sistem: ot molekuly k organizmu. Minsk:BEELSENS; 2002. 63-72. (in Russian)
Kaler GV, Melnikova AM, Matus VK, Konev SV. Vzaimodeistvie ozona s membranami eritrotctcitov. Biologicheskie membrany. 1989;6(11):1164-9. (in Russian)
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