Physical mechanisms of interaction of actinocin derivatives with DNA. 5. Spectrophotometric analysis of dimerization of actinocin derivatives with different length of side chaines
Keywords:
actinocin antibiotics, dimerization, thermodynamic parameters, caffeineAbstract
Dimerization of synthetic phenoxazone antibiotics, derivatives of actinocin, with methylene chains of different length in the side groups of the chromophores, has been studied by UV-visible spectrophotometry as a function of ionic strength and temperature of the solution. Absorption spectra in the monomer and dimer forms of antibiotics and dimerization constants at different temperatures have been determined using the program of optimization of spectrophotometric concentration dependences. It has been shown that aggregation of the investigated antibiotics depends on the salt concentration in solution and is substantially higher in solutions of large ionic strength. The tendency in the change of enthalpy and entropy of dimerization of molecules in non-salt aqueous solution has been found as a function of the number of methylene groups in the phenoxazone antibiotic. Using the calculated values of dimerization constants and molar extinction coefficients in the monomer and dimer forms of antibiotic with two methylene groups in the side chain, parameters of its complexation with caffeine have been determined.
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