Physical mechanisms of interaction of actinocin derivatives with DNA 10. Physical properties of a complex of actinocil-bis-(3-dimethylaminopropyl)amine and double-stranded polyribocytidylic acid by methods of Raman spectroscopy and spectrophotometry

  • Yu.N. Bliznyuk Institute for Radiophysics and Electronics, NASU
  • T.V. Bolbukh Institute for Radiophysics and Electronics, NASU
  • E.B. Kruglova Institute for Radiophysics and Electronics, NASU
  • M.A. Semenov Institute for Radiophysics and Electronics, NASU
  • V.Ya. Maleev Institute for Radiophysics and Electronics, NASU
Keywords: Raman spectroscopy, spectrophotometry, actinocin derivative ActIII, polyribocytidylic acid ро1у(С),ро1у(С*), double-stranded structure, complexing, aggregates

Abstract

A study of complexation of actinocin derivative actinocil-bis-(3-dimethyl aminopropyl)amine (ActIII) with a double-stranded structure of a polyribocytidylic acid ро1у(С),ро1у(С*) in aqueous solution was carried out by the methods of spectrophotometry and Raman spectroscopy. The quantitative analysis of the spectrophotometric concentration dependences allowed us to establish P/D intervals (P is the moles of phosphate, D is the moles of ligand), where ligands prevail with the different types of binding to double-stranded ро1у(С),ро1у(С*) structure (monomeric binding and aggregating). Groups of atoms of ActIII ligand phenoxazone ring participating in complexing with a polynucleotide matrix by the external type of binding were determined on the basis of obtained Raman spectra of ActIII- ро1у(С),ро1у(С*) complexes.

 

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Author Biographies

Yu.N. Bliznyuk, Institute for Radiophysics and Electronics, NASU

12 Acad. Proscura str., Kharkov, 61085, Ukraine

T.V. Bolbukh, Institute for Radiophysics and Electronics, NASU

12 Acad. Proscura str., Kharkov, 61085, Ukraine

E.B. Kruglova, Institute for Radiophysics and Electronics, NASU

12 Acad. Proscura str., Kharkov, 61085, Ukraine

M.A. Semenov, Institute for Radiophysics and Electronics, NASU

12 Acad. Proscura str., Kharkov, 61085, Ukraine

V.Ya. Maleev, Institute for Radiophysics and Electronics, NASU

12 Acad. Proscura str., Kharkov, 61085, Ukraine

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Published
2005-06-06
Cited
How to Cite
Bliznyuk, Y., Bolbukh, T., Kruglova, E., Semenov, M., & Maleev, V. (2005). Physical mechanisms of interaction of actinocin derivatives with DNA 10. Physical properties of a complex of actinocil-bis-(3-dimethylaminopropyl)amine and double-stranded polyribocytidylic acid by methods of Raman spectroscopy and spectrophotometry. Biophysical Bulletin, 1(15), 29-34. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/18000