Decondensation of chromatin state in human sperm cells after cryopreservation and electromagnetic irradiation of millimeter range
Keywords:
cryopreserved human sperm, electromagnetic irradiation, motility, chromatin, apoptosisAbstract
In the work investigated the effects of electromagnetic irradiation of millimeter range of 0.03 mW/cm2
density on cryopreserved human sperm in normal and pathological state. Morpho-functional state of sperm before and after irradiation was assessed using light, fluorescence microscopy and FACS analysis. Application of irradiation of the samples for 15 minutes at normospermia and 5 minutes at asthenozoospermia resulted in a significant increase in 7AAD+sperm about the appropriate value in the control group. It has been found that electromagnetic irradiation of millimeter range of 0.03 mW/sm2 density on cryopreserved human sperm for 5 minutes at normospermia and 15 minutes at asthenozoospermia increases the fraction of rectilinearly moving sperm without changing the integrity of membrane, nuclear chromatin state and generation of apoptosis.
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