Free radical lipid peroxidation, pyrene and 1,8-anilinenapthalenesulfonate fluorescence in rat liver microsomes under the influence of γ-irradiation and alimentary factors
Abstract
There was studied the interference of single γ-irradiation (8 Gr dose) and unbalanced (with animal proteins and antioxidant series vitamins) food allowance and alimentary addition (black chokeberry) on pyrene, 1,8-ANS (monomer and excimer) fluorescence intensity and ascorbic Fe2+-induced lipids peroxidation (LP) intensity and on lipid hydroperoxides contents in rat liver microsomes. It was determined, that γ-irradiation of animals fed with unbalanced food allowance leads to more acute form of LP activation and microsomal membranes reconstruction with following negative charged group number decrease on membrane surface, than irradiation of rats fed with standard vivarium food allowance. The alimentary addition of black chokeberry graded LP activation and charged groups distribution changes on liver microsomes surface of irradiated rats fed with unbalanced food. Obtained data allows us to conclude that black chokeberry alimentary addition has an antioxidant and membrane protective action and it's capable to correct liver microsomes detoxication ability changes of irradiated organism, especially, irradiated animals fed with unbalanced food allowance.
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