Dielectric permittivity of rabbit erythrocytes under type 2 diabetes and application of glucose-lowering agents at 9.2 ghz
Keywords:
UHF dielectrometry, erythrocytes, permittivity, diabetes, hydration
Abstract
This paper covers the effect of different methods of pharmacological correction of the experimental type
2 diabetes on the dielectric characteristics of rabbit erythrocytes. In the experiments dexamethasone model
of diabetes was used. As samples packed rabbit erythrocytes of intact animals, packed rabbit erythrocytes
treated with water (placebo group), and packed rabbit erythrocytes treated with glucose-lowering agents –
metformin and the new experimental compound 1408 were used. Using the method of UHF dielectrometry
at the frequency of 9.2 GHz values of the real ε' and imaginary ε'' parts of the complex permittivity of
packed rabbit erythrocytes have been obtained. An increase in the average values of the dielectric loss ε'',
in the static dielectric permittivity εs of erythrocytes, as well as an increase in the dispersion of these
parameters in the group of rabbits with diabetes without correction compared with the controlled group.
When treated with the compound 1408 no reduction of the parameters was observed. When using metformin for the correction of diabetes the tendency to the decrease in average values of ε', ε'', and εs erythrocytes is observed, as well as in the dispersion of these parameters in comparison with the group of rabbits with diabetes without correction. Group of rabbits which received metformin only were not significantly different from the controlled group in the parameters ε'' and εs.
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References
1. American Association of Clincal Endocrinologists. Medical Guidelines for Clinical Practice for the Management of Diabetes Mellitus: AACE Diabetes Mellitus Clinical Practice Guidelines Task Force // Endocrine Practice. – 2007. – Vol. 13, Suppl. 1. – P. 3-66.
2. Izmenenija fiziko-himicheskih svojstv jeritrocitov pri nekotoryh onkologicheskih zabolevanijah / T.V. Sviridova, T.A. Voroncov, B.D. Rjabov, [i dr.] // Pat. fiziol. - 1983. - №6. - S. 3-5.
3. Morfometricheskoe sostojanie jeritrocitov i funkcional'noe sostojanie ih membran u bol'nyh lekarstvennoj bolezn'ju / Je.N. Soloshenko, A.E. Dunaeva, E.M. Mamotjuk, [i dr.] // Eksperymental'na i klinichna medycyna. - 2001. - №3. - S. 67-72.
4. Kolmakov V.N. Opredelenie pronicaemosti jeritrocitarnyh membran v dinamike hronicheskih zabolevanij pecheni / V.N. Kolmakov // V kn. Hronicheskij gepatit i cirroz. – L., 1988. – S. 26-28.
5. Pronicaemost' membran jeritrocitov u bol'nyh s infekcionnoj patologiej / O.I. Kulapina, V.F. Kirichuk, I.A. Utc, [i dr.] // Serija. Kriticheskie tehnologii. Membrany. – 2005. – №1 (25). – S. 3-11.
6. Bolis L. Membranes and disease / L. Bolis, J.F. Hoffman, A. Leaf - N. York, 1980. - 410 р.
7. Babu N. Influence of hyperglycemia on aggregation, deformability and shape parameters of erythrocytes / N. Babu, M. Singh // Clin Hemorheol. Microcirc. – 2004. – Vol. 31, № 4. – P. 273-280.
8. Structural alterations of the erythrocyte membrane proteins in diabetic retinopathy / I.K. Petropoulos, P.I. Margetis, M.H. Antonelou, [et al.] // Graefes Arch. Clin. Exp. Ophthalmol. – 2007. – Vol. 245, № 8. – P. 1179-1188.
9. Kagava Ja. Biomembrany / Ja. Kagava – M.: Vysshaja shkola. - 1985. – 303 s.
10. Shamsiev D.F. Reologicheskie svojstva jeritrocitov u bol'nyh s gnojno-vospalitel'nymi zabolevanijami nosa i okolonosovyh pazuh / D.F. Shamsiev // Vestnik otolaring. – 2001. – №1. – S. 22-23.
11. Issledovanie gidratacii membran jeritrocitov do i posle luchevoj terapii / L.V. Batjuk, S.V. Gatash, O.A. Gorobchenko, O.T. Nikolov // Biofizicheskij vestnik. – 2003. – Vyp. 2 (13). – S. 72-78.
12. Aksenov S.I. Voda i ee rol' v reguljacii biologicheskih processov / S.I. Aksenov. – Moskva-Izhevsk: Institut komp'juternyh issledovanij, 2004. – 212 s.
13. Trincher K.S. O strukturirovannoj vode, prilegajuwej k poverhnostnomu sloju jeritrocitov. // Sb. Sostojanie i rol' vody v biologicheskih ob#ektah / K.S. Trincher; [Pod red. L.P. Kajushina]. – M.: Nauka, 1967. – S. 143-150.
14. Stabilizirujuwaja rol' primembrannyh slojov v podderzhanii strukturnoj celostnosti kletochnyh membran / Ja.Ju. Komissarchik, S.V. Levin, B.E. Sviridov, I.Ju. Sabolauskas // Obwie mehanizmy kletochnyh reakcij na povrezhdajuwee dejstvie. - L.: Nauka, 1977. - S. 29-31.
15. Hydration forces between phospholipid bilayers / R.P. Rand, V.A. Parsegian // Biochim. Biophys. Acta. - 1989. - Vol. 988, №3. - P. 351-376.
16. Gennis R. Biomembrany: Molekuljarnaja struktura i funkcii / R. Gennis; [Per. s angl]. – M.: Mir, 1997. – 624 s.
17. Schepps J.L., Foster K.R. The UHF and microwave dielectric properties of normal and tumour tissues: variation in dielectric properties with tissue water content / J.L. Schepps, K.R. Foster // Phys. Med. Biol. – 1980. V. 25, No 6. – P. 1149-1159.
18. Metod jelektronnogo paramagnitnogo rezonansa dlja kontrolja za sostojaniem uglevodnogo obmena i jeffektivnosti insulinoterapii detej, bol'nyh saharnym diabetov / L.L. Vahrusheva, A.G. Maksina, Ju.A. Knjazev, [i dr.] // Rossijskij vestnik perinatologii i pediatrii. – 1999. – №3. – S. 49-51.
19. Sostojanie gidratacii plazmy krovi i jeritrocitov u beremennyh s neoslozhnennym gestacionnym processom i anemiej / I.I. Nikiforovich, A.N. Ivanjan, A.V. Litvinov, [i dr.] // Rossijskij vestnik akushera-ginekologa. – 2009. – № 2. – S. 4-7.
20. Poltorak V.V., Gorbenko N.Y. Eksperymental'ne vyvchennja novyh gipoglikemichnyh zasobiv: metod. rekomendacii' / V.V. Poltorak, N.Y. Gorbenko; M-vo ohorony zdorov’ja Ukrai'ny, Akademija medychnyh nauk. – K.: 2001. – S. 396-408. – (Doklinichni doslidzhennja likars'kyh zasobiv; za red. O. V. Stefanova).
21. Nikolov O.T. Izmerenie kompleksnoj dijelektricheskoj pronicaemosti zhidkih dijelektrikov s bol'shimi poterjami / O.T. Nikolov, T.A. Zhiljakova // Zhurnal fizicheskoj himii. – 1991. – T. 65, №5. – S. 1312-1316.
22. Jenin Pierre C. Some observations on the dielectric properties of hemoglobin’s suspending medium inside human erythrocytes / Pierre C. Jenin, H.P. Schwan // Biophys. J. – 1980. – V. 3. – P. 285-294.
23. Debaj P. Poljarnye molekuly / P. Debaj – M-L.: 1931. – 245 s.
24. Suzuki M. Hydration Study of Proteins in Solution by Microwave Dielectric Analysys / M. Suzuki, J. Shigematsu, T. Kodama // J. Phys. Chem. – 1996. – V. 100. – P. 7279-7282.
25. Levin R.L. Effect of hydration on the water content of human erythrocytes / R.L. Levin, E.G. Cravalho, C.E. Huggin // Biophys. J. – 1976. – V.16. – P. 1411-1426.
26. Mashimo S. Dielectric relaxation time and structure of bound water in biological materials / S. Mashimo, Sh. Kuwabara // J. Phys. Chem. – 1987. – V. 91, No 25. - P. 6337-6338.
27. Harakteristiki izmenenij v krovi ryb pod dejstviem himicheskogo zagrjaznenija sredy po dannym nizkotemperaturnoj 1N-JaMR-spektroskopii / A.S. Gorjunov, G.A. Suhanova, A.G. Borisova, V.S. Sidorov // Biopolimery i kletka. – 1996. – T. 12, №6. – S. 1-5.
28. Rozhkov S.P. Trehkomponentnaja sistema voda-biopolimer – iony kak model' molekuljarnyh mehanizmov osmoticheskogo gomeostaza / S.P. Rozhkov // Biofizika. – 2001. – T. 46, № 1. – S. 53-59.
29. DiBiasio A. D-glucose-induced alteration in the electrical parameters of human erythrocyte cell membrane / A. DiBiasio, C. Cametti // Bioelectrochemistry. – 2010. – V. 77. – P. 151-157.
30. Dielectric spectroscopy study of specific glucose influence on human erythrocyte membranes / Y. Hayashi, L. Livshits, A. Caduff, Y. Feldman // J. Phys. D: Appl. Phys. – 2003. – V. 36. – P. 369–374.
31. Desouky O.S. Rheological and electrical behavior of erythrocytes in patients with diabetes mellitus / O.S. Desouky // Romanian J. Biophys. - 2009. - Vol. 19, No. 4. - P. 239–250.
32. Sostojanie citoplazmaticheskih membran pri jeksperimental'nom saharnom diabete / N.P. Mikajeljan, Ju.A. Knjazev, A.E. Gurina, [i dr.] // Saharnyj diabet. – 1999. - № 3 (4). – S. 48-51.
33. Balabolkin M.I. Rol' okislitel'nogo stressa v patogeneze sosudistyh oslozhnenij saharnogo diabeta i primenenie vitaminov i mikrojelementov dlja ih lechenija i profilaktiki / M.I. Balabolkin // Klinicheskaja jendokrinologija. - 2006. - № 6. - S. 1-7.
34. Gorbenko N.I., Gorshunskaja M.Ju. Metformin – sredstva vybora dlja preduprezhdenija i umen'shenija vyrazhennosti serdechno-sosudistoj patologii u bol'nyh saharnym diabetom 2-go tipa / N.I. Gorbenko, M.Ju. Gorshunskaja // Ukrai'ns'kyj medychnyj chasopys. – 2005. - № 4 (48). – S. 29-36.
2. Izmenenija fiziko-himicheskih svojstv jeritrocitov pri nekotoryh onkologicheskih zabolevanijah / T.V. Sviridova, T.A. Voroncov, B.D. Rjabov, [i dr.] // Pat. fiziol. - 1983. - №6. - S. 3-5.
3. Morfometricheskoe sostojanie jeritrocitov i funkcional'noe sostojanie ih membran u bol'nyh lekarstvennoj bolezn'ju / Je.N. Soloshenko, A.E. Dunaeva, E.M. Mamotjuk, [i dr.] // Eksperymental'na i klinichna medycyna. - 2001. - №3. - S. 67-72.
4. Kolmakov V.N. Opredelenie pronicaemosti jeritrocitarnyh membran v dinamike hronicheskih zabolevanij pecheni / V.N. Kolmakov // V kn. Hronicheskij gepatit i cirroz. – L., 1988. – S. 26-28.
5. Pronicaemost' membran jeritrocitov u bol'nyh s infekcionnoj patologiej / O.I. Kulapina, V.F. Kirichuk, I.A. Utc, [i dr.] // Serija. Kriticheskie tehnologii. Membrany. – 2005. – №1 (25). – S. 3-11.
6. Bolis L. Membranes and disease / L. Bolis, J.F. Hoffman, A. Leaf - N. York, 1980. - 410 р.
7. Babu N. Influence of hyperglycemia on aggregation, deformability and shape parameters of erythrocytes / N. Babu, M. Singh // Clin Hemorheol. Microcirc. – 2004. – Vol. 31, № 4. – P. 273-280.
8. Structural alterations of the erythrocyte membrane proteins in diabetic retinopathy / I.K. Petropoulos, P.I. Margetis, M.H. Antonelou, [et al.] // Graefes Arch. Clin. Exp. Ophthalmol. – 2007. – Vol. 245, № 8. – P. 1179-1188.
9. Kagava Ja. Biomembrany / Ja. Kagava – M.: Vysshaja shkola. - 1985. – 303 s.
10. Shamsiev D.F. Reologicheskie svojstva jeritrocitov u bol'nyh s gnojno-vospalitel'nymi zabolevanijami nosa i okolonosovyh pazuh / D.F. Shamsiev // Vestnik otolaring. – 2001. – №1. – S. 22-23.
11. Issledovanie gidratacii membran jeritrocitov do i posle luchevoj terapii / L.V. Batjuk, S.V. Gatash, O.A. Gorobchenko, O.T. Nikolov // Biofizicheskij vestnik. – 2003. – Vyp. 2 (13). – S. 72-78.
12. Aksenov S.I. Voda i ee rol' v reguljacii biologicheskih processov / S.I. Aksenov. – Moskva-Izhevsk: Institut komp'juternyh issledovanij, 2004. – 212 s.
13. Trincher K.S. O strukturirovannoj vode, prilegajuwej k poverhnostnomu sloju jeritrocitov. // Sb. Sostojanie i rol' vody v biologicheskih ob#ektah / K.S. Trincher; [Pod red. L.P. Kajushina]. – M.: Nauka, 1967. – S. 143-150.
14. Stabilizirujuwaja rol' primembrannyh slojov v podderzhanii strukturnoj celostnosti kletochnyh membran / Ja.Ju. Komissarchik, S.V. Levin, B.E. Sviridov, I.Ju. Sabolauskas // Obwie mehanizmy kletochnyh reakcij na povrezhdajuwee dejstvie. - L.: Nauka, 1977. - S. 29-31.
15. Hydration forces between phospholipid bilayers / R.P. Rand, V.A. Parsegian // Biochim. Biophys. Acta. - 1989. - Vol. 988, №3. - P. 351-376.
16. Gennis R. Biomembrany: Molekuljarnaja struktura i funkcii / R. Gennis; [Per. s angl]. – M.: Mir, 1997. – 624 s.
17. Schepps J.L., Foster K.R. The UHF and microwave dielectric properties of normal and tumour tissues: variation in dielectric properties with tissue water content / J.L. Schepps, K.R. Foster // Phys. Med. Biol. – 1980. V. 25, No 6. – P. 1149-1159.
18. Metod jelektronnogo paramagnitnogo rezonansa dlja kontrolja za sostojaniem uglevodnogo obmena i jeffektivnosti insulinoterapii detej, bol'nyh saharnym diabetov / L.L. Vahrusheva, A.G. Maksina, Ju.A. Knjazev, [i dr.] // Rossijskij vestnik perinatologii i pediatrii. – 1999. – №3. – S. 49-51.
19. Sostojanie gidratacii plazmy krovi i jeritrocitov u beremennyh s neoslozhnennym gestacionnym processom i anemiej / I.I. Nikiforovich, A.N. Ivanjan, A.V. Litvinov, [i dr.] // Rossijskij vestnik akushera-ginekologa. – 2009. – № 2. – S. 4-7.
20. Poltorak V.V., Gorbenko N.Y. Eksperymental'ne vyvchennja novyh gipoglikemichnyh zasobiv: metod. rekomendacii' / V.V. Poltorak, N.Y. Gorbenko; M-vo ohorony zdorov’ja Ukrai'ny, Akademija medychnyh nauk. – K.: 2001. – S. 396-408. – (Doklinichni doslidzhennja likars'kyh zasobiv; za red. O. V. Stefanova).
21. Nikolov O.T. Izmerenie kompleksnoj dijelektricheskoj pronicaemosti zhidkih dijelektrikov s bol'shimi poterjami / O.T. Nikolov, T.A. Zhiljakova // Zhurnal fizicheskoj himii. – 1991. – T. 65, №5. – S. 1312-1316.
22. Jenin Pierre C. Some observations on the dielectric properties of hemoglobin’s suspending medium inside human erythrocytes / Pierre C. Jenin, H.P. Schwan // Biophys. J. – 1980. – V. 3. – P. 285-294.
23. Debaj P. Poljarnye molekuly / P. Debaj – M-L.: 1931. – 245 s.
24. Suzuki M. Hydration Study of Proteins in Solution by Microwave Dielectric Analysys / M. Suzuki, J. Shigematsu, T. Kodama // J. Phys. Chem. – 1996. – V. 100. – P. 7279-7282.
25. Levin R.L. Effect of hydration on the water content of human erythrocytes / R.L. Levin, E.G. Cravalho, C.E. Huggin // Biophys. J. – 1976. – V.16. – P. 1411-1426.
26. Mashimo S. Dielectric relaxation time and structure of bound water in biological materials / S. Mashimo, Sh. Kuwabara // J. Phys. Chem. – 1987. – V. 91, No 25. - P. 6337-6338.
27. Harakteristiki izmenenij v krovi ryb pod dejstviem himicheskogo zagrjaznenija sredy po dannym nizkotemperaturnoj 1N-JaMR-spektroskopii / A.S. Gorjunov, G.A. Suhanova, A.G. Borisova, V.S. Sidorov // Biopolimery i kletka. – 1996. – T. 12, №6. – S. 1-5.
28. Rozhkov S.P. Trehkomponentnaja sistema voda-biopolimer – iony kak model' molekuljarnyh mehanizmov osmoticheskogo gomeostaza / S.P. Rozhkov // Biofizika. – 2001. – T. 46, № 1. – S. 53-59.
29. DiBiasio A. D-glucose-induced alteration in the electrical parameters of human erythrocyte cell membrane / A. DiBiasio, C. Cametti // Bioelectrochemistry. – 2010. – V. 77. – P. 151-157.
30. Dielectric spectroscopy study of specific glucose influence on human erythrocyte membranes / Y. Hayashi, L. Livshits, A. Caduff, Y. Feldman // J. Phys. D: Appl. Phys. – 2003. – V. 36. – P. 369–374.
31. Desouky O.S. Rheological and electrical behavior of erythrocytes in patients with diabetes mellitus / O.S. Desouky // Romanian J. Biophys. - 2009. - Vol. 19, No. 4. - P. 239–250.
32. Sostojanie citoplazmaticheskih membran pri jeksperimental'nom saharnom diabete / N.P. Mikajeljan, Ju.A. Knjazev, A.E. Gurina, [i dr.] // Saharnyj diabet. – 1999. - № 3 (4). – S. 48-51.
33. Balabolkin M.I. Rol' okislitel'nogo stressa v patogeneze sosudistyh oslozhnenij saharnogo diabeta i primenenie vitaminov i mikrojelementov dlja ih lechenija i profilaktiki / M.I. Balabolkin // Klinicheskaja jendokrinologija. - 2006. - № 6. - S. 1-7.
34. Gorbenko N.I., Gorshunskaja M.Ju. Metformin – sredstva vybora dlja preduprezhdenija i umen'shenija vyrazhennosti serdechno-sosudistoj patologii u bol'nyh saharnym diabetom 2-go tipa / N.I. Gorbenko, M.Ju. Gorshunskaja // Ukrai'ns'kyj medychnyj chasopys. – 2005. - № 4 (48). – S. 29-36.
Cited
How to Cite
Soloviov, D. V., Gorobchenko, O. A., Shatalova, T. A., Ovsyannikova, T. M., Gladkyh, A. I., Nikolov, O. T., & Gatash, S. V. (1). Dielectric permittivity of rabbit erythrocytes under type 2 diabetes and application of glucose-lowering agents at 9.2 ghz. Biophysical Bulletin, 2(27). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2528
Section
Cell biophysics
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