The influence of congelation and gamma-irradiation on cord blood serum through the data of VHF-dielectrometry

O. A. Gorobchenko V.N. Karazin Kharkiv National University
N. T. Markova Institute for Problems of Cryobioloby and Cryomedicine, NAS of Ukraine
A V. Adeljanov V.N. Karazin Kharkov National University
O. T. Nikolov V.N. Karazin Kharkov National University
E. O. Nardid Institute for Problems of Cryobioloby and Cryomedicine, NAS of Ukraine
S. V. Gatash V.N. Karazin Kharkov National University
O. A. Nardid V.N. Karazin Kharkov National University

Keywords: cord blood serum, gamma-irradiation, low temperatures, permittivity, VHF-dielectrometry

Abstract

Influence of freezing to -196°C and subsequent gamma-irradiation with doses 20, 50, 100, and 150 Gy on human cord blood serum by method VHF-dielectrometry have been studied in this work. Cord blood serum was frozen at the rate of 300 400°C/min to the temperature of -196°С by immersing it in liquid nitrogen. Native specimens and specimens after freezing-thaw were irradiated at room temperature in an air atmosphere with gamma - rays source Co⁶⁰. Measurement of the real part of frozen, irradiated, and subsequently frozen and irradiated serum specimens complex permittivity ε' was done by the cavity method on frequency 9,3 GHz at room temperature (20°С). As the results of the investigation done freezing of cord blood serum to -196°C was lead to the result of the increase of permittivity what may be serum proteins cryoaggregation. On the contrary, gamma-irradiation leads to decrease cord blood serum permittivity. Decrease of dielectric parameters of serum after irradiation is assumed to be due to conformational changes in its proteins connected with break-in intramolecular hydrogen bonds, loosening in macromolecules surface and increasing amount of hydration water owing to the formation of new hydrogen bonds with volume water molecules. Magnitudes of the real part of complex permittivity ε' of the cord blood after freezing to 196°C and subsequent gamma-irradiation have lower values than those just after freezing but higher than those only after gamma-irradiation. It justifies to the additive contribution of these physical factors in the magnitude of permittivity of the cord blood serum. Diversities of these magnitudes of permittivity of the serum after freezing and serum after subsequent action of freezing and gamma-irradiation are coincide within the limits of error and are independent of the dose of irradiation showing independent nature of the action of low temperatures and irradiation with doses up to 150 Gy on properties of cord blood serum.

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Author Biographies

O. A. Gorobchenko, V.N. Karazin Kharkiv National University

4 Svobody Sq., Kharkov, 61077

N. T. Markova, Institute for Problems of Cryobioloby and Cryomedicine, NAS of Ukraine

23 Pereyaslavskaya St., Kharkov, 61015

e-mail: olga_bio_f@yahoo. com

A V. Adeljanov, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

O. T. Nikolov, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

E. O. Nardid, Institute for Problems of Cryobioloby and Cryomedicine, NAS of Ukraine

23 Pereyaslavskaya St., Kharkov, 61015

e-mail: olga_bio_f@yahoo. com

S. V. Gatash, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

O. A. Nardid, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077

References

1. Мошко Ю.А. Применение криоконсервированной сыворотки кордовой крови в лечении женщин с хроническими салпингоофоритами, // Проблемы криобиологии. - 2001. -№ 1. - С. 70-75.

2. Грищенко В.І., Прокопюк О.С., Ліпіна О.В., Мошко Ю.О. Заготівля, кріоконсервування сироватки і плазми кордової крові та іх клінічне застосування. Методичні рекомендації. - Харків, 2000. - 11с.

3. House С, House J.A., Yedloutschnig R.J. Inactivation of viral agents in bovine serum by gamma irradiation. // Can J Microbiol. - 1990. - V. 36. - P. 737-740.

4. Хенли Э., Джонсон Э. Радиационная химия. -M.: Атомизцат, 1974. -415 с.

5. Hackl E.V., Gatash S.V., Níkolov O.T. Using UHF-dielectrometry to study protein structural transitions.
// J. Biochem. Biophys. Meth. - 2005. - V. 63, № 2. - P. 137-148.

6. Горобченко O.A., Мошко Ю.А., Николов О.Т., Нардид O.A., Липина О.В., Гаташ С.В. Влияние режима замораживания на диэлектрические свойства сыворотки кордовой крови // Проблемы криобиологии. - 2004. - № 2. - С. 4-10.

7. Марковский А.Л. Влияние факторов криоконсервации на гидратацию глобулярных белков: Автореф. дис. ... канд. биол. наук: 03.00.22. - Харьков, 1984. - 20 с.

8. Нардид Э.О., Науменко Е.И., Розанова С.Л., Нардид O.A. Влияние режимов замораживания на агрегацию белков сыворотки кордовой крови человека // Проблемы криобиологии. - 2006. - Т. 16, № 3. -С. 311-315.

9. Горобченко O.A. Влияние γ-облучения и температуры на конформацию белков крови. Дис... канд. физ.-мат. наук: 03.00.02. - Харьков, 2005. - 150 с.

10. Горобченко O.A., Николов О.Т., Нардид O.A. Исследование влияния γ-облучения на
структуру бычьего сывороточного альбумина методом спиновых меток // Прикладная
радиоэлектроника. - 2004, - Т. 2, № 2. - С. 84-87.

11. Горобченко O.A., Николов О.Т., Гаташ С. В. Влияние γ-облучения на термоиндуцированные конформационные переходы и гидратацию фибриногена. // Біополімери і
клітина. - 2006. - T. 22, № 2. - С. 162-165.

12. Шарпатый В. А. Радиационная химия биополимеров. - М.: Энергоиздат, 1981. - 168 с.

13. Sunae Moon, Kyung Bin Song. Effect of gamma-irradiation on the molecular properties of egg white proteins. // Food Sei. Biotechnol. - 2000. - V. 9, №4. - P. 239-242.