Decondensation of chromatin state in human sperm cells after cryopreservation and electromagnetic irradiation of millimeter range
Keywords:
cryopreserved human sperm, electromagnetic irradiation, motility, chromatin, apoptosis
Abstract
In the work investigated the effects of electromagnetic irradiation of millimeter range of 0.03 mW/cm2
density on cryopreserved human sperm in normal and pathological state. Morpho-functional state of sperm before and after irradiation was assessed using light, fluorescence microscopy and FACS analysis. Application of irradiation of the samples for 15 minutes at normospermia and 5 minutes at asthenozoospermia resulted in a significant increase in 7AAD+sperm about the appropriate value in the control group. It has been found that electromagnetic irradiation of millimeter range of 0.03 mW/sm2 density on cryopreserved human sperm for 5 minutes at normospermia and 15 minutes at asthenozoospermia increases the fraction of rectilinearly moving sperm without changing the integrity of membrane, nuclear chromatin state and generation of apoptosis.
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References
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3. Бецкий О. В. Современные представления о механизмах воздействия низкоинтенсивных миллиметровых волн на биологические объекты / О. В. Бецкий, Н. Н. Лебедева // Мат. междунар. симп. «Миллиметровые волны в медицине и биологии» Москва, 1999. – С.130–133. /Betskiy O. V. Sovremennyie predstavleniya o mehanizmah vozdeystviya nizkointensivnyih millimetrovyih voln na biologicheskie ob'ekty / O. V. Betskiy , N. N. Lebedeva // Mat. mezhdunar. simp. «Millimetrovyie volnyi v meditsine i biologii» Moskva. – 1999. – S. 130–133./
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7. Мужское бесплодие и нарушение структурной организации хроматина сперматозоидов. Существует ли связь? / О. А. Воробьева, А. В. Воскресенская, А. А. Одинцов [и др] // Проблемы репродуктологии. – 2005. – №6. – С.56–62. /Muzhskoe besplodie i narushenie strukturnoy organizacii hromatina spermatozoidov. Sushchestvuet li svyaz? / O.A.Vorobieva, A.V. Voskresenskaya, Odincov A.A. [i dr] // Problemy reproduktologii. – 2005. – №6. – S. 56–62./
8. Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients / F. Brugnon, E. Van Assche, G. Verheyen [et al] // Human Reproduction. - 2006. - №21. – P.683-685.
9. Barroso G. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa / G. Barroso, M. Morshedi, S. Oehninger // Hum. Reprod. – 2000. – №15. – P.1338 – 1344.
10. DNA integrity in human spermatozoa relationships with semen quality / D. S. Irvine, J. P. Twigg, E. L. Gordon [et al] // Journal of Andrology. – 2000. – №21. – P.33–44.
11. Sun J. G. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro / J. G. Sun, R. F. Jurisicova, A. Casper // Biology of Reproduction. – 1997. – №56. – P.602–607.
12. Integrating new tests of sperm genetic integrity into semen analysis: breakout group discussion / S. D. Perreault, R. J. Aitken, H. W. G. Baker [et al] // Adv. Exp. Med. Boil. – 2003. – V.518. – P. 253—268.
13. Blanc-Layrac G. Morphological and biochemical analysis of cell death inhuman ejaculated spermatozoa / G. Blanc-Layrac, A. F. Bringuier, R Guillot // Cell Mol Biol. – 2000. – V.46. –№ 1. – P. 187–197.
14. Apoptosis and necrosis in human ejaculated spermatozoa / N. Lachaud, J. Tesarik, M. L. Canadas [et al] // Hum Reprod.-2004. – V.19. – P. 607-610.
15. Schlegel P. N. Yet another test of sperm chromatin structure / P. N. Schlegel, D. A. Paduch // Fertil Steril. – 2005. – V.84. – P.854-859.
16. Seli E. Spermatozoal nuclear determinants of reproductive outcome: implications for ART / E. Seli, D. Sakkas // Human Reproduction. – 2005. – №11. – P.337-349.
17. 7-Aminoactinomycin D for apoptosis staining in flow cytometry / N. Zembruski, V. Stache, W. Haefeli, J. Weiss // Analytical Biochemistry. – 2012. –V. 429. – P.79–81.
Cited
How to Cite
VolkovaN. О., PavlovichО. V., Gapon, G. O., & Nikolov, O. T. (1). Decondensation of chromatin state in human sperm cells after cryopreservation and electromagnetic irradiation of millimeter range. Biophysical Bulletin, 2(30). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2318
Section
Action of physical agents on biological objects
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