IR-SPECTROSCOPIC STUDY OF Cu2+ ION INTERACTION WITH DNA IN AQUEOUS SOLUTIONS CONTAINING ADDITIONS OF 1,2-PROPANEDIOL AND GLYCEROL
Abstract
The present work studied the Cu2+ ion interaction with DNA in aqueous solutions containing low (up to 20 v %) additions of glycerol or 1,2-propanediol at 29 and 45°C by IR-spectroscopy. Cu2+ ions were shown to induce the DNA transition into compact form in aqueous and water-glycerol (propanediol) solutions both at 29 and at 45°C. This process is of high positive cooperativity. 1,2-propanediol added to the DNA aqueous solution increases cooperativity of the DNA transition into compact state and decreases the Cu2+ ions concentration required to induce DNA compactisation. The 1,2-propanediol effect increases moriotonously with the rise of its volume concentration (in the concentration range 0-20 v %) at 29 and 45°C. In contrast to propanediol, the glycerol effect on DNA compactisation is of non-monotonous character: small (4 v %) glycerol additions to DNA aqueous solution decrease cooperativity of the DNA transition into compact state at 29°C. Comparison of effects of small additions of 1,2-propanediol and glycerol for which dielectric permeability of solution changes practically the same, on the Cu2+-induced DNA condensation, permits the conclusion that the DNA condensation induced by the Cu2+ ions depends not only on dielectric permeability but on the structure of the solution formed.
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