Mg2+ ion effect on stability of double-stranded polynucleotide formed by polyriboinosinic and polyribocytidilic chains
Abstract
Mg2+ effect on the thermal stability of the double-stranded polynucleotide Poly I •Poly C (IC) under conditions close to physiological ones (0.1 M Na+, pH 7) was studied by the differential UV spectroscopy method. The initial increase of the ion concentration ([Mg2+]) leads to the rise of the melting temperature Tm and to the decrease of the absorption hyperchromicity value, conditioned by the heating-induced helix-coil transition (hmax). The last effect is supposed to be a result of the Mg2+ interaction with π-electrons of hypoxanthine or cytosine rings (cation-π-interaction). When [Mg2+] reaches its critical value ([Mg2+]cr ∼ 10-4M), Tm decreases cooperatively and hmax rises up to values close to those observed in the absence of magnesium. It is supposed that at [Mg2+] = [Mg2+]cr IC transits into a new structural state. With [Mg2+]>[Mg2+]cr Tm of the polynucleotide increases again and hmax lowers slightly.
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References
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