Theoretical analysis of the efficiency of linear and two-step freezing regimens for mammalian embryo cryopreservation
Keywords:
cryopreservation, cooling rate, rapid two-step freezing, mammalian embryo
Abstract
The research was aimed at the theoretical evaluation of the application expediency of non-linear two-step freezing regimens for mammalian embryo cryopreservation. Basing on the comparison of theoretical dependency of intracellular solution overcooling on time (or temperature) under linear and two-step freezing regimens there the advantages of the latter for mammalian embryo cryopreservation were established and explained. Theoretical evaluation of optimal cooling rate by the two-factor cryodamage theory for two-cell murine embryos coincides with experimental data
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References
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3. Mazur P. Cryobiology: the freezing of biological objecls//Science.-1970.-168. N3934/-p.939-949.
4. Котык А., Яначек К. Мембранный транспорт -М;Мир.-1980.-341с.
5. Wood M.J., Farrant J. Preservation mouse embryos by two-step frrezing//Cryobiology.-1980.-17-p. 178-180.
Published
2008-09-29
Cited
How to Cite
Ostankov, M. V. (2008). Theoretical analysis of the efficiency of linear and two-step freezing regimens for mammalian embryo cryopreservation. Biophysical Bulletin, 1(20), 73-78. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/1583
Section
Biophysics of complex systems
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