Indicator dye behavior in the system glucose oxidase + glucose
Keywords:
glucose biosensor, glucose oxidase, indicator dyes
Abstract
Using the absorption spectroscopy technique the group of indicator dyes including bromocresol purple, phenol red, neutral red, auramine O and acridine orange has been examined from the viewpoint of the dye applicability to the design of optical glucose biosensors. The absorbance changes of bromocresol purple, phenol red and neutral red observed in the glucose oxidase + glucose system were interpreted ui terms of the dye sensitivity to the pH reduction occurring while glucose is catalytically oxidized. The arguments in favor of neutral red interaction with glucose oxidase are obtained.
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References
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Vo-Dinh T, Cullum B. Fresenius J. Anal. Chem. 2000;366:540-51.
Fraser DM. Medical Device Technology. 1994;5:44-7.
Ravi Shankaran D, Uehara N, Kato K. Biosensors and Bioelectronics. 2003;18:721-8.
Bianco P. Reviews in Molecular Biochnology. 2002;82:393-409.
Rosenzweig Z, Kopelman R. Anal. Chem. 1996;68:1408-13.
Mehrvar M, Bis C, Scharer JM, Moo-Young M, Luong JH. Analytical Sciences. 2000;16:677-92.
Potyrailo RA, Hobbs SE, Hieftje GM. Fresenius J. Anal. Chem. 1998;362:349-73.
Mchedlov-Petrossyan NO, Isaenko YuV, Salamanova NV, Alekseeva VL, Savvina LP. Journal of Analytical Chemistry. 2003;58:1018-30.
Gorbenko GP, Mchedlov-Petrossyan NO, Chernaya TA. J. Chem. Soc., Faraday Trans. 1998;94:2117-25.
Published
2018-06-25
Cited
How to Cite
Gorbenko, G. P., & Domanov, Y. A. (2018). Indicator dye behavior in the system glucose oxidase + glucose. Biophysical Bulletin, 2(13), 116-119. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12470
Section
Methods of biophysical investigations
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