Protolytic behavior of indicator dye in the model membrane systems. I. Dye partitioning into lipid phase

  • L. V. Budko V.N. Karazin Kharkov National University
  • G. P. Gorbenko V.N. Karazin Kharkov National University
  • V. M. Ioffe V.N. Karazin Kharkov National University
Keywords: protolytic equilibria, indicator dye, partition coefficient, lipid bilayer

Abstract

Protolytic and partition equilibria of indicator dyes in the model lipid systems have been analyzed. A methodological approach has been developed allowing the partition coefficients of the protonated and deprotonated dye forms to be derived from spectrophotometric measurements. The most effective ways of employing the indicator dyes for monitoring the changes in the lipid bilayer properties have been suggested.

Downloads

Download data is not yet available.

Author Biographies

L. V. Budko, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077, Ukraine

G. P. Gorbenko, V.N. Karazin Kharkov National University

 4 Svobody Sq., Kharkov, 61077, Ukraine

V. M. Ioffe, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077, Ukraine

References

White S, Ladokhin A, Jayasinghe S, Hristova K. J. Biol. Chem. 2001;276:32395-8.

Mouritsen O. Current Opin. Colloid Interface Sci. 1998;3:78-87.

Kinnunen PKJ. Chem. Phys. Lipids. 1996;81:151-66.

Carnie S, McLaughlin S. Biophys. J. 1983;44:325-32.

Hendrich AB, Michalak K. Current Drug Targets. 2003;4:23-30.

Roux M, Newmann Y, Hodges R. Biochem. 1989;28:2313-21.

Dempsey C, Bitbol M, Watts A. Biochem. 1989;28:6590-5.

Kleinschmidt J, Mahaney J, Thomas D, Marsh D. Biophys. J. 1997;72:767-78.

Schwarz G, Beschiachvili G. Biochim. Biophys. Acta. 1989;979:82-90.

Sankaram M, Marsh D. Elsevier. 1993;127-62.

Dumas F, Lebrun M, Peyron P, Lopez A, Tocanne J. Biochim. Biophys. Acta. 1999;1421:295-305.

Babin Y, D’Amour J, Pigeon M, Pezolet M. Biochim. Biophys. Acta. 1987;903:78-88.

Moller J, Kragh-Hansen U. Biochem. 1975;14:2317-23.

Mashimo T, Uede I. Proc. Natl. Acad. Sci. USA. 1979;76:5114-8.

Gorbenko G, Mchedlov-Petrossyan N, Chernaya T. J. Chem. Soc. Faraday Trans. 1998;94:2117-25.

Cevc G. Biochim. Biophys. Acta. 1990;1031:311-82.

Tocanne J, Teissie J. Biochim. Biophys. Acta. 1990;1031:111-42.
Published
2007-06-05
Cited
How to Cite
Budko, L. V., Gorbenko, G. P., & Ioffe, V. M. (2007). Protolytic behavior of indicator dye in the model membrane systems. I. Dye partitioning into lipid phase. Biophysical Bulletin, 1(18), 48-52. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12620

Most read articles by the same author(s)