General antioxidant activity at the allotransplantation of embryonal muscle tissue in rats
Abstract
Allotransplantation of embryonal tissue is one of the actual directions in modern theoretical and medical biochemistry which is developed for stimulation and restoration of functions of the organism. Allotransplantation of the embryonal tissue is considered as possible alternative to traditional conservative methods of treatment, and also as methodological basis of experimental development. The purpose of the work was to investigate the effect of allotransplantation of the embryonic femoral muscle tissue on the general antioxidant activity. For allotransplantation of the embryonal muscle tissue 2–3 weeks' embryos were used. Under ether anesthesia in aseptic conditions the animal was fixed on surgical board in dorsal decubitus, the surgery field was shaved and processed by antiseptic agent three times. The section was carried out on internal average third of hip. Femoral muscle tissue was taken from embryos and then fixed by ligature to the hip of adult rat. The wound was sewn up layer-by-layer with densely noose suture. Used allotransplantation was carried out according to surgical rules of operations on muscles. The studied indicators were determined on the first, third and seventh days after the operative intervention in donor and recipient tissues. The implantation of the formed tissue and the false operation were performed in order to rule out the effect of both the operation itself and the effect of tissue implantation, in order to detect the effect of embryonic tissue on the formed. We examined each indicator not only when implanting the embryonic tissue, but also with the false operation and implantation of the formed tissue to the formed one, and compared the indices for all these three types of intervention. The established changes in the general antioxidant activity for the third day of the study of allotransplantation of the femoral muscle tissue of the embryo indicate a significant decrease in the overall antioxidant activity in the recipient's tissue, which may be due to the breakdown of antioxidant defense, characterized by the development of free radical damage to various components of the cell and tissues, which is a syndrome of peroxidation, but by the seventh day the antioxidant systems are restored.
Downloads
References
Лебедева А.И., Муслимов С.А., Мусина Л.А., Щербаков Д.А. Регенерация скелетной мышечной ткани экспериментальных животных, индуцированная биоматериалом Аллоплант // International Journal of Experimental Education. – 2014. – C. 68–71. /Lebedeva A.I., Muslimov S.A., Mussina L.A., Shcherbakov D. A. Regeneration of skeletal muscle tissue of experimental animals induced by biomaterial Alloplant // International Journal of Experimental Education. – 2014. – No. 3. – P. 68–71./
Литтманн И. Оперативная хирургия. – Будапешт: Издательство академии наук Венгрии, 1985. – С. 1055–1056. /Littmann I. Operative surgery. – Budapest: Publishing house of the Academy of Sciences of Hungary, 1985. – P. 1055–1056./
Овсяннікова Л.М., Чумак А.А., Коваленко О.М. та ін. Антиоксидантна система, окисна модифікація білків і ліпідів в розвитку порушень життєдіяльності у віддаленому періоді після Чорнобильської аварії // Медичні наслідки аварії на Чорнобильській атомній електростанції. – К.: ДІА, 2007. – С. 422–436. /Ovsyannikova L.M., Chumak A.A., Kovalenko O. M. et al. Antioxidant system, oxidative modification of proteins and lipids in development of life activity disturbances in the remote period after Chernobyl accident // Medical effects of accident on Chernobyl nuclear power station. – Kyiv.: DIA, 2007. – P. 422–436./
Почерняева В.Ф., Цебржинский О.И., Шиш Н.В. Определение источников активных форм кислорода // Буковинський медичний вісник. – 2005. – Т.9, №2. – С. 214–215. /Pochernyaeva V.F., Tsebrzhinsky O.I., Shish N.V. Definition of reactive oxygen species sources // Bukovinian Medical Herald Journal. – 2005. – Vol.9, no. 2. – P. 214–215./
Репин В.С. Медицинская клеточная биология: новые фундаментальные и прикладные исследования // Трансплантация фетальных тканей и клеток человека. – М., 1996. – С. 19–27. /Repin V.S. Medical cell biology: new basic and applied researches // Transplantation of fetal tissues and human cells. – Moscow, 1996. – P. 19–27./
Станков Д.С., Катунян П.И., Крашенинников Н.Е. Нейротрансплантация в лечении травмы спинного мозга // Вестник трансплантологии и искусственных органов. – 2003. – №1. – С. 44–45. /Stankov D.S., Katunyan P.I., Krasheninnikov N.E. Neurotransplantation in treatment of spinal cord injury // Russian Journal of Transplantology and Artificial Organs. – 2003. – No. 1. – P. 44–45./
Тесевич Л.И., Барьяш В.В. Пластическое возмещение дефектов и деформаций челюстно-лицевой области свободной пересадкой тканей. – Минск: БГМУ, 2010. – 63с. /Tesevich L.I., Baryash V.V. Plastic compensation of defects and deformations of maxillofacial area by free tissue grafting. – Minsk: BSMU, 2010. – 63p./
Шумаков В.И., Онищенко Н.А., Крашенинников Н.Е. Костный мозг как источник получения мезенхимальных клеток // Вестник трансплантологии и искусственных органов. – 2002. – №4. – С. 3–6. /Shumakov V.I., Onishchenko N.A., Krasheninnikov N.E. Bone marrow as source for receiving mesenchymal cells // Russian Journal of Transplantology and Artificial Organs. – 2002. – No. 4. – P. 3–6./
Ghaly A., Marsh D.R. Aging-associated oxidative stress modulates the acute inflammatory response in skeletal muscle after contusion injury // Exp. Gerontol. – 2010. – Vol.45. – P. 381–388.
Gierer P., Rother J., Mittlmeier T. et al. Ebselen reduces inflammation and microvascular perfusion failure after blunt skeletal muscle injury of the rat // J. Trauma. – 2010. – Vol.68. – P. 853–858.
Kozakowska M., Pietraszek-Gremplewicz K., Jozkowicz A., Dulak J. The role of oxidative stress in skeletal muscle injury and regeneration: focus on antioxidant enzymes // J. Muscle Res. Cell Motil. – 2015. – Vol.36 (6). – P. 377–393.
Saclier M., Cuvellier S., Magnan M. et al. Monocyte/macrophage interactions with myogenic precursor cells during skeletal muscle regeneration // FEBS J. – 2013. – Vol.280. – P. 4118–4130.
Singh S., Canseco D.C., Manda S.M. et al. Cytoglobin modulates myogenic progenitor cell viability and muscle regeneration // Proc. Natl. Acad. Sci. USA. – 2014. – Vol.111. – P. 129–138.
Authors retain copyright of their work and grant the journal the right of its first publication under the terms of the Creative Commons Attribution License 4.0 International (CC BY 4.0), that allows others to share the work with an acknowledgement of the work's authorship.
