Content of vitamine C metabolites in rats organs at acute blood loss
Abstract
There has been studied the effect of acute blood loss, which was modeled by a single loss of 30% of the circulating blood, on the fluctuations in the content of ascorbic (AA), dehydroascorbic (DAA), diketogulonic (DKGA) acid and their sum in the organs of rats in dynamics for the fifth, twelfth, nineteenth and twenty-sixth days after the blood loss. Acute blood loss caused a significant decrease in the content of all parameters of the system of metabolites of ascorbic acid – their sum, AA, DAA and DKGA – by 10–73 % compared to the control. The most significant decrease was in the content of AA, which was not restored in all organs until the end of the study period. The DAA content in all organs increased from the 12th day, and then decreased during the experiment. The content of the DKGA increased from the 19th day of the experiment. At the same time, it was found that on 26th day in the kidneys, the DAA content exceeded the control value by 42%, and the content of DKGA in the liver and blood – by 25–60 %. The content of the amount of ascorbic acid metabolites at the end of the experiment was almost restored, but this recovery occurred in various ways: in the kidneys – due to an increase in the DAA content, in other organs – by increasing the concentration of DKGA. The parts of AA from the sum of acids (in %) after blood loss significantly decreased, starting from the 5th day, and the process of its recovery began to occur only after the 19th day. The ratio of the amount of the vitamin component of the acids of the ascorbic acid system to the content of the non-vitamin DKGA was increased in the kidneys on the 12th and 26th days of the experiment, in other organs this index decreased 2.3–3.1 times in comparison with the control. The obtained data can be explained by the increased consumption of ascorbic acid to neutralize the effects of the intensification of oxidative processes under oxidative stress, which were activated by the action of acute blood loss, due to its reversible conversion to dehydroascorbic acid, and the latter irreversibly to diketogulonic acid.
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