Chemical characteristics of peripheral blood granulocyte biomembranes in frequently ill children with autonomic dysfunction syndrome
Abstract
The problem of frequently ill children has extreme relevance in medical practice. A special group is FIC with autonomic dysfunction syndrome, born prematurely with perinatal damage of the central nervous system (CNS). In previous studies, we found that FIC who were born prematurely with perinatal damage of the central nervous system exhibit lower immunoreactivity than their healthy peers. Purpose of the work – to study the biochemical indicator of immunocompetent cells (the study of the phospholipid composition of granulocyte membranes) of the frequently ill children with autonomic dysfunction syndrome, born prematurely with perinatal damage to the central nervous system (CNS). Materials and methods. 68 children in the age of 6-7 years who are belonging to the group of frequently ill were examined. Out of 68 children, there were 36 children with autonomic dysfunction syndrome (SVD) born prematurely with perinatal hypoxic-ischemic damage to the central nervous system (group 1) and 32 children without autonomic dysfunction syndrome born on time without central nervous system damage (group 2). Results. A study of the phospholipid composition of blood granulocyte membranes of patients of the studied groups revealed significant differences comparison with the control group. The revealed changes in granulocyte biomembranes were more pronounced for FIC with SVD born prematurely with perinatal CNS damage than for children born on time without CNS and SVD damage. Conclusions. The obtained data allow us to conclude that FIL who were born preterm with perinatal damage of the central nervous system, changes in the biochemical characteristics of membranes are observed to a slightly greater extent than for children who were born on time without central nervous system damage. The revealed changes in immunocompetent cells are characterized by an increase in the polarity of the lipid component of biomembranes and a decrease in the relative content of neutral lipids in them. These violations lead to a change in protein-lipid interactions, which, as a result, leads to a change in the microviscosity of biomembranes and their structural and functional properties. At these children forme an inferior immune response to an infectious agent.
Downloads
References
Lukashchuk SV, Lemko OІ. Parts of and recurrent respiratory infections in children: current problems (review). Problems of Clinical Pediatrics. 2016; 3-4: 66-71. https://doi.org/10.31655/2307-3373-2018-4-7-11
Lukashchuk SV, Lemko OI. Principles of management of children with frequent and recurrent illness of airways (review). Modern pediatrics. 2016; 7: 48–52. https://doi.org/10.15574/SP.2016.79.48
Moiseenko RO, Dudina OO, Goyda NG. Analysis of the incidence and prevalence of diseases in children in Ukraine for the period 2011-2015. Modern pediatrics. 2017; 2 (82): 17–27. https://doi.org/10.15574/SP.2017.82.17
Reggie HC Lee, Michelle HH Lee, Celeste YC Wu. Cerebral ischemia and neuroregeneration. Neural Regen Res. 2018;13(3):373–385. https://doi.org/10.4103/1673-5374.228711.
Popov NN, Olenich VB, Savvo AN. Cytokine status and balance of cells of the immune system in children with autonomic dysfunction syndrome born prematurely with perinatal lesions of the central nervous system (CNS). Modern scientific bulletin. 2014; 31 (227): 49 - 56.
Popov NN, Olenich VB, Ognivenko YeV, Savvo AN. Clinical manifestations of vegetative dysfunction syndrome in children with chronic purulent sinusitis В: Modern scientific potential - 2017: materials of the XIII International scientific and practical Conference; 2017 Feb 8 - Mar 7; Sheffield. Sheffield, England; 2017. P. 3−6.
Popov NN, Piantkovskaya OV, Olenych VB, Savvo ON, Solovyova AG. The level of cytokines and blood serum adhesion factor in bronchial asthma in children. In: International Academy Jornal Web of Scholar, No. 9 (18), Vol.1, 30 leaf fall, 2017 rock.: abstracts. Dubai, UAE, 2017. P. 72–76.
Popov NN, Piantkovskaya OV, Olenych VB, Savvo ON, Ognivenko YeV. Neuroadaptogenics in children who have undergone perinatal hypoxic-ischemic lesions of the central nervous system. In: International Trends in Science and Technology: Proceedings of the XIV International Scientific and Practical Conference; 2019 June 30; Warsaw. Warsaw, Poland; 2019. P. 34–37.
Senchuk VV. A course of lectures on biochemistry. Minsk: BSU, 2005. Part 1. Biomolecules. P. 137-139.
Severin SYe, Soloveva TA. Biochemistry workshop: practical. manual. Moscow: Moscow State University, 1989. 509 pp.
Kanskaya NV, Ivanov VV, Stepovaya YeA. Modification of the method for determining lipids. Siberian Medical Journal. 2014; 29 (1): 69–73. https://doi.org/10.29001/2073-8552-2014-29-1-69-73
Vladimirov YuA, Dobretsov TYe. Fluorescent probes in biological membranes research: Pract. manual. Moscow: Science, 1980. 320 pp.
Boulgouris NV, Plataniotis KN. Biometrics: Theory, Methods, and Applications. Hoboken, NJ: Wiley, 2009. 763 p.
Meloun M, Militky J. Statistical Data Analysis: A Practical Guide. Woodhead Publishing, 2011. 800 p.
Popov NN, Ognivenko YeV. Physicochemical and biological properties of membranes of neutrophilic granulocytes of patients with chronic purulent maxillary sinusitis suffering from diabetes mellitus. Experimental and Clinical Medicine. 2007; 3: 8-12
