Computational Study of Drug Delivery Systems with Radionuclide and Fluorescence Imaging Modalities. II. Doxorubicin Delivery Systems Based on Albumin and Transferrin

  • V. Trusova Department of Medical Physics and Biomedical Nanotechnologies, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-7087-071X
  • U. Malovytsia Department of Medical Physics and Biomedical Nanotechnologies, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0002-7677-0779
  • P. Kuznietsov O.I. Akhiezer Department for Nuclear Physics and High Energy Physics, V.N. Karazin Kharkiv National University, Kharkiv, Ukraine https://orcid.org/0000-0001-8477-1395
  • I. Karnaukhov National Science Center “Kharkov Institute of Physics and Technology”, Kharkiv, Ukraine
  • A. Zelinsky National Science Center “Kharkov Institute of Physics and Technology”, Kharkiv, Ukraine https://orcid.org/0000-0002-4110-8523
  • B. Borts National Science Center “Kharkov Institute of Physics and Technology”, Kharkiv, Ukraine https://orcid.org/0000-0002-1492-4066
  • I. Ushakov National Science Center “Kharkov Institute of Physics and Technology”, Kharkiv, Ukraine
  • L. Sidenko National Science Center “Kharkov Institute of Physics and Technology”, Kharkiv, Ukraine
  • G. Gorbenko National Science Center “Kharkov Institute of Physics and Technology”, Kharkiv, Ukraine https://orcid.org/0000-0002-0954-5053
DOI: https://doi.org/10.26565/2312-4334-2025-1-46
Keywords: Drug delivery nanosystems, Human serum albumin, Transferrin, Doxorubicin, Technetium complexes, Fluorescent dyes, Molecular docking

Abstract

This study explores the development of advanced protein-based drug delivery systems for doxorubicin (DOX), an anticancer agent, incorporating both radionuclide (technetium-99m complexes) and fluorescence (Methylene Blue (MB), Indocyanine Green (IG), cyanine AK7-5 and squaraine SQ1) imaging modalities. Building upon previous research on albumin-based carriers, this work expands the scope by introducing transferrin (TRF) as a complementary protein component to create a more sophisticated and targeted delivery platform. Molecular docking technique was employed to design and characterize the multimodal delivery systems that incorporate radiopharmaceuticals and near-infrared fluorescent dyes. The results demonstrate that technetium-99m-based radiopharmaceuticals are capable of strong noncovalent binding to human serum albumin (HSA) and its complexes with transferrin. A comprehensive analysis of docking scores and interacting amino acid residues reveals that HSA-TRF-TcHyn/TcMEB/TcDIS-DOX-IG/SQ1 systems show the highest potential for experimental testing and further development. These findings support the potential of HSA-TRF complexes as nanocarriers with dual imaging capabilities for DOX delivery, offering an approach to enhance therapeutic efficacy while reducing systemic toxicity in anticancer treatment.

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Published
2025-03-03
Cited
How to Cite
Trusova, V., Malovytsia, U., Kuznietsov, P., Karnaukhov, I., Zelinsky, A., Borts, B., Ushakov, I., Sidenko, L., & Gorbenko, G. (2025). Computational Study of Drug Delivery Systems with Radionuclide and Fluorescence Imaging Modalities. II. Doxorubicin Delivery Systems Based on Albumin and Transferrin. East European Journal of Physics, (1), 376-382. https://doi.org/10.26565/2312-4334-2025-1-46
Issue
No. 1 (2025): East European Journal of Physics
Section
Original Papers

Copyright (c) 2025 V. Trusova, U. Malovytsia, P. Kuznietsov, I. Karnaukhov, A. Zelinsky, B. Borts, I. Ushakov, L. Sidenko, G. Gorbenko

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