Complex formation of aromatic biologicaly active compounds with antibiotic topotecan: analysis by means of 1h nuclear magnetic resonance spectroscopy

  • A. A. Mosunov Sevastopol National Technical University
  • A. S. Buchelnikov Sevastopol National Technical University
  • M. P. Evstigneev Sevastopol National Technical University
Keywords: topotecan, complexation, heteroassociation, interceptor, protector mechanisms

Abstract

In the present work with use of 1H NMR spectroscopy data the analysis of heteroassociation of antibiotic
topotecan (TPT) with aromatic biologically active compounds (BAC): caffeine, mutagens ethidium
bromide and proflavine, antibiotic daunorubicin, vitamins flavin-mononucleotide and nicotinamide, is
carried out. The analysis was performed taking into account the existence of the antibiotic in aqueous
environment under physiological pH in two various forms – lactone and carboxylate. Equilibrium
constants of heteroassociation and induced chemical shifts of TPT protons (in lactone and carboxylate
forms) and the BACs within the complexes were obtained. It is found that the complex formation TPTBАС is characterized by the stacking of the chromophores and additionally stabilized in case of
proflavine by intermolecular hydrogen bond

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Author Biographies

A. A. Mosunov, Sevastopol National Technical University

99053, Sevastopol, Ukraine

A. S. Buchelnikov, Sevastopol National Technical University

99053, Sevastopol, Ukraine

M. P. Evstigneev, Sevastopol National Technical University

99053, Sevastopol, Ukraine

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Cited
How to Cite
Mosunov, A. A., Buchelnikov, A. S., & Evstigneev, M. P. (1). Complex formation of aromatic biologicaly active compounds with antibiotic topotecan: analysis by means of 1h nuclear magnetic resonance spectroscopy. Biophysical Bulletin, 1(26). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2547