Fluorescence quenching in protein-lipid system: contribution of diffusion-controlled factors

  • Ya. A. Domanov Karazin Kharkiv National University
  • G. P. Gorbenko Karazin Kharkiv National University https://orcid.org/0000-0002-0954-5053
  • O. V. Gamolina Karazin Kharkiv National University

Анотація

Using the model of diffusion-controlled reactions between asymmetric species the quenching of the protein fluorescence by extrinsic quenchers has been considered. The changes in bimolecular quenching rate constant stemming from the protein immobilization on the surface of lipid vesicles have been evaluated. It has been shown that association of the proteins whose radius is less than 2 nm with lipid vesicles can be followed by the apparent decrease of the quenching efficiency due to reduced translational and rotational diffusion coefficients of the bound protein.

Завантаження

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Біографії авторів

G. P. Gorbenko, Karazin Kharkiv National University

4 Svobody Sq., Kharkiv 61077, Ukraine

O. V. Gamolina, Karazin Kharkiv National University

4 Svobody Sq., Kharkiv 61077, Ukraine

Посилання

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Erdey-Gruz T. Transport phenomena in aqueous solutions. New York:John Wiley and Sons, Inc; 1974.

Gamolina OV, Gorbenko GP, Domanov YeA. Biophysical Bulletin. 2002;10(1):124-6.
Опубліковано
2018-06-25
Цитовано
Як цитувати
Domanov, Y. A., Gorbenko, G. P., & Gamolina, O. V. (2018). Fluorescence quenching in protein-lipid system: contribution of diffusion-controlled factors. Біофізичний вісник, 1(12), 117-119. вилучено із https://periodicals.karazin.ua/biophysvisnyk/article/view/12153
Розділ
Методи біофізичних досліджень