The influence of actinocine derivative ACT III on melting of DNA in the DNA ACT III complexes at different ligand concentrations

  • E. G. Bereznyak Institute for Radiophysics and Electronics of NAS of Ukraine
  • E. B. Kruglova Institute for Radiophysics and Electronics of NAS of Ukraine
  • A. S. Khrebtova Institute for Radiophysics and Electronics of NAS of Ukraine
  • Ye. V. Dukhopelnykov Institute for Radiophysics and Electronics of NAS of Ukraine
  • A. V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine
Keywords: DNA, actinocine derivative, differential scanning calorimetry, spectrophotometry, multimodal binding, thermodynamics

Abstract

Spectrophotometric concentration dependencies of the system of calf thymus DNA and actinolite derivative Act III in 0.015 M Na + solution are analyzed. The information about binding modes and stoichiometry of complexes is obtained. Thermodynamic parameters of DNA in the complexes are found by means of differential scanning calorimetry technique. It is shown that two significantly thermodynamically and structurally different types of complexes can be formed depending on the ratio of polynucleotide-to-ligand concentrations (P/D). At low P/D values Act III binds preferentially to the sugar-phosphate backbone and forms a non-sequence-specific complex. At a high P/D ratio, the ligand intercalates into DNA and changes the structure of GC-rich blocks. In the intermediate P/D range both types of binding coexist. Melting of complexes results in redistribution of ligand and increase of the Act III concentration in the complex by aggregate type. It was found that the size of the binding sites depends on the experimental technique employed.

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Author Biographies

E. G. Bereznyak, Institute for Radiophysics and Electronics of NAS of Ukraine

12 Ac. Proskura Str., Kharkiv, 61085, Ukraine

 

E. B. Kruglova, Institute for Radiophysics and Electronics of NAS of Ukraine

12 Ac. Proskura Str., Kharkiv, 61085, Ukraine

 

A. S. Khrebtova, Institute for Radiophysics and Electronics of NAS of Ukraine

12 Ac. Proskura Str., Kharkiv, 61085, Ukraine

Ye. V. Dukhopelnykov, Institute for Radiophysics and Electronics of NAS of Ukraine

12 Ac. Proskura Str., Kharkiv, 61085, Ukraine

A. V. Zinchenko, Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine

23 Pereyaslavskaya Str., Kharkiv, 61015

References

Vardevanyan P.O., Antonyan A.P., Manukyan G.A., Karapetyan A.T. // Experimental and Molecular Medicine. 2001. Vol.33. No.4. P.205-208.

Barcelo F., Capo D., Portugal J. // Nucleic Acids Research. 2002. Vol.30. No.20. P. 4567-4573.

Barcelo P., Orliz-lombatdia M., Portugal J. // Biochimica et Biophysica Acta. 2001. Vol.1519. P.175-184.

Sovenyhazy K., Bolderon J., Petty J. // Nucleic Acids Research. 2003. Vol.31. No. 10. P. 2561-2569.

Карапетян А.Т., Пермогоров В.И., Франк-Каменетский М.Д., Лазуркин Ю.С. // Молекулярная биология. 1972. Т.6. Вып.6. С.867-874.

Borisova O.F., Shchyolkina A.K., Karapetyan A.T., Surovaya A.N. // Mol. Biology. 1998. Vol.32. No.5. P.718-724.

Snyder J.G., et al. // Proc.Natl.Acad.Sci.USA. 1989. Vol.86. P.3968-3972.

Yoo H., Rill R.L. // J.Mol.Recognit. 2001. 14. P.145-150.

Chen F.-M, et al. // BiophysJ. 2003. Vol.84. P.432-439.

Chin K.-H., et al. // Nucleic Acids Research. 2003. Vol.31. No.10. P. 2622-2629.

Круглова Е.Б., Гладковская Н.А. // "Вісник Харківського Університету" №637, 'Біофізичний Вісник". 2004. Вып. 1-2 (14).С.43-47

Anti-cancer Drug Design: Biological and Biophysical Aspects of Synthetic Phenoxazone Derivatives. Eds Veselkov A.N., Davies D.B. Sevastopol: SEVNTU Press. 2002. 259 р.

Круглова Е.Б., Гладковская Н.А., Малеев В.Я. // Биофизика. 2005. Т. 50. Вып.2. С. 253-264.

Круглова Е.Б., Малеев В.Я., Глибин Е.Н., Веселков А.Н. // 'Вісник Харківського Університету" №560,'Біофізичний Вісник". 2002. Вып.1 (10). С. 12-20.

Hinz H.-J., Schwarz F.P. // Pure Appl. Chem., Vol. 73, No. 4, pp. 745-759, 2001

Круглова Е.Б., Ермак Е.Л. // "Вісник Харківського Університету" №560, 'Біофізичний Вісник". 2003. Вып. 1(12). С. 64-69

Esposito D., Del Vicchio P., Barone G. // J.Am.Chem.Soc. 1997. Vol.119. No. 11. P.2606-2613.

Marky L.A., Blumenfeld K.S., Breslauer K.J. // Nucleic Acids Research. Vol. 11, No.9, 1983, 2857-2870.

Marky L.A., Snyder J.G., Breslauer K.J. // Nucleic Acids Research. 1983. Vol.11. No. 16. P.5701-5715.

Ахрем А.А., Ландо Д.Ю. //Молекулярная биология. 1981. Т. 15. №5. С.1083-1091.

Chaires J.B. // Archives of Biochemistry and Biophysics. 2006. 453. P.24-29.

Chaires J.B. // Biopolymers. 1998. Vol. 44. Issue 3. P.201-215.

Scott E.V., Jones R.L., Banville D.L., et al. // Biochemistry. 1988. Vol.27. No.3. P.915-923.

Scott E.Z., Zon G., Marzilli L.G., Wilson W.D. // Biochemistry. 1988. Vol.27. No.20. P.7940-7951.

Благой Ю.П., Галкин В.Л., Гладченко Г.О., Корнилова СВ., Сорокин В.А., Шкорбатов А.Г. Металлокомплексы нуклеиновых кислот в растворах. К. Наукова думка. 1991. 270с.

Березняк Е.Г., Круглова Е.Б. и др. II Биофизика. 2007. Т.52. Вып.5. С.812-821.

Ландо Д.Ю., Иванова М.А., Ахрем А.А. // Молекулярная биология. 1980. Т.14. Вып.6. С. 1281-1288.

Published
2007-06-05
Cited
How to Cite
Bereznyak, E. G., Kruglova, E. B., Khrebtova, A. S., Dukhopelnykov, Y. V., & Zinchenko, A. V. (2007). The influence of actinocine derivative ACT III on melting of DNA in the DNA ACT III complexes at different ligand concentrations. Biophysical Bulletin, 2(19), 29-35. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/17112