Physical mechanisms of interaction of actinocin derivatives with DNA. 7. Piezogravimetric study of DNA complexes with actinocin derivatives
Keywords:
DNA, actinocin derivatives, intercalation, piezogravimetry, D'Arsi-Watt model, thermodynamical parameters of adsorptionAbstract
The hydration isotherms of thymus DNA, actinomycin derivatives with a different number of methylene groups in side chains, and their complexes are received by a quartz crystal microbalance. It is found that the sorption ability of water molecules for complexes is less than for free DNA and depends on the length of side methylene chains: complexes with longer side chains of ligand adsorb a smaller number of water molecules. Experimental hydration isotherms were compared with model isotherms obtained by the modified D'Arsi-Watt equation. It allows us to determine the dependence of the constant of water-binding with complexes on the length of methylene chains. It is found that the binding constants for complexes are greater than ones for free DNA. The heats of water adsorption on DNA and its complexes at a different level of adsorption are calculated. It is shown that the interaction with water molecules gives an appreciable contribution (50-60 %) to the stabilization energy of DNA in complexes.
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