Spectroscopic and calorimetris investigation of flavin-mononucleotide - DNA system
Keywords:
flavin - mononucleotide, DNA, calorimetry, spectrophotometry, binding parameters
Abstract
The interaction of flavin – mononucleotide (FMN) with salmon testes DNA is investigated by differential
scanning calorimetry, spectrophotometry, and fluorescent spectroscopy. It is shown that the addition of FMN to
DNA doesn't change DNA melting parameters significantly. The binding constant K = 50 М-1 for the
interaction of FMN with DNA is calculated from spectrophotometric data. Such value of the binding constant
is incomparably smaller than binding constants for the biologically active ligand to DNA and the constants for
ligand - FMN heteroassotiation.
Downloads
Download data is not yet available.
References
1. L. Vergani, M. Barile, C. Angelini, A.B. Burlina, L. Nijtmans, M.P. Freda, C. Brizio, E. Zerbetto, F. Dabbeni -Sala. Riboflavin therapy. Biochemical heterogeneity in two adult lipid storage myopathies// Brain. - 1999. - Vol. 122. - №. 12. – Р. 2401-2411.
2. M.P. Evstigneev, Yu.V. Mykhina, D.B. Davies. Complexation of daunomycin with a DNA oligomer in the presence of an aromatic vitamin (B2) determined by NMR spectroscopy// Biophys. Chemist. – 2005. - V. 118. - P. 118–127.
3. M.P. Evstigneev, Yu.V. Mukhina, D.B. Davies. 1H NMR study of thehetero-association of flavin-mononucleotide with mutagenic dyes: ethidium bromide and proflavine// Mol. Phys. – 2006. – V. 104. – P. 647–654.
4. A.N. Veselkov, M.P. Evstigneev, A.O. Rozvadovska, Yu.V. Mukhina, D.B.Davies. A structural and thermodynamic analysis of novatrone and flavin mononucleotide heteroassociation in aqueous solution by 1H NMR spectroscopy// Russ. J. Bioorgan. Chem. – 2005.-V. 31.- P. 453–459.
5. A.N. Veselkov, M.P. Evstigneev, A.O. Rozvadovskaya, Yu.V. Mukhina, K.A. Rybakova, D.B. Davies, 1H NMR analysis of the complex formation of aromatic molecules of antibiotic and vitamin in aqueous solution: Heteroassociation of actinomycin D and flavin mononucleotide// Biophysics. -2005.-V. 50. - P. 20–27.
6. D.J. Patel, A.K. Suri. Structure, recognition and discrimination in RNA aptamer complexes with cofactors, amino acids, drugs and aminoglycoside antibiotics // J.Biotechnol. - 2000. - V. 74(1). - P. 39-60.
7. M.P. Evstigneev, Yu.V. Mukhina, D.B. Davies. Complexation of daunomycin with a DNA oligomer in the presence of an aromatic vitamin (B2) determined by NMR spectroscopy// Biophys. Chemist. -2005.-V. 118.-P. 118–127.
8. N. Dardare, M.S. Platz. Binding Affinities of Commonly Employed Sensitizers of Viral Inactivation// Photochemistry and Photobiology. – 2002. - V. 75(6). - P. 561–564.
9. K. Kuratomi, Y. Kobayashi. Studies on the interactions between DNA and flavins// Biochem. Biophys. Acta.- 1977. –V. 476.- P. 207–217.
10. Е.Г. Березняк, Н.А. Гладковская, Е.В. Духопельников, A.C. Хребтова, А.В. Зинченко. Особенности взаимодействия профлавина с ДНК при различных соотношениях их концентраций// Биофизика. -2009. – T. 54. - В.5. - C. 805-812.
11. F. Barcelо, D. Capо, J. Portugal. Thermodynamic characterization of the multivalent binding of chartreusin to DNA// Nucleic Acids Research. – 2002. - Vol. 30. - №. 20. - Р. 4567-4573.
12. H. Grajek, I. Gryczynski, P. Bojarski, Z. Gryczynski, Sh. Bharill, L. Kułak. Flavin mononucleotide fluorescence intensity decay in concentrated aqueous solutions// Chemical Physics Letters. – 2007. – V. 439. - Р. 151–156.
13. J. T. M. Kennis, S. Crosson, M. Gauden, I. H. M. van Stokkum, K. Moffat, R. van Grondelle Primary Reactions of the LOV2 Domain of Phototropin, a Plant Blue-Light Photoreceptor// Biochemistry. - 2003. - V. 42 (12). – Р. 3385-3392.
14. А.Т. Карапетян, П.О. Вардеванян, М.Д. Франк-Каменецкий. Влияние концентрации ионов на теплоту перехода спираль-клубок ДНК// Биополимеры и клетка.- 1989. - Т.5. - № 5. - С. 31-37.
15. A.T. Karapetian, P.O. Vardevanian, M.D. Frank-Kamentskii. Enthalpy of Helix-Coil Transition of DNA Depence on Na+ Concentration and GC Content// J. Biomol. Struct. Dyn. – 1990. - V. 8(1). - P. 131-138.
16. J.D.McGhee, P.H. von Hippel. Theoretical aspects of DNA-Protein interactions: Cooperative and non-cooperative binding of large ligands to a one-dimensional homogeneous lattice// J. Mol. Biol. - 1974. – V. 86. - P. 469-489.
17. J.B. Chaires. Energetics of drug-DNA interactions// Biopolymers.- 1997. – V. 44(3). - P. 201-15.
18. L.-P. Lin, L.-S. Huang, C.-W. Lin, C.-K. Lee, J.-L. Chen, S.-. Hsu, Sh. Lin. Determination of Binding Constant of DNA-binding Drug to Target DNA by Surface Plasmon Resonance Biosensor Technology// Current Drug Targets - Immune, Endocrine & Metabolic Disorders. - 2005. - V. 5. - P. 61-72.
2. M.P. Evstigneev, Yu.V. Mykhina, D.B. Davies. Complexation of daunomycin with a DNA oligomer in the presence of an aromatic vitamin (B2) determined by NMR spectroscopy// Biophys. Chemist. – 2005. - V. 118. - P. 118–127.
3. M.P. Evstigneev, Yu.V. Mukhina, D.B. Davies. 1H NMR study of thehetero-association of flavin-mononucleotide with mutagenic dyes: ethidium bromide and proflavine// Mol. Phys. – 2006. – V. 104. – P. 647–654.
4. A.N. Veselkov, M.P. Evstigneev, A.O. Rozvadovska, Yu.V. Mukhina, D.B.Davies. A structural and thermodynamic analysis of novatrone and flavin mononucleotide heteroassociation in aqueous solution by 1H NMR spectroscopy// Russ. J. Bioorgan. Chem. – 2005.-V. 31.- P. 453–459.
5. A.N. Veselkov, M.P. Evstigneev, A.O. Rozvadovskaya, Yu.V. Mukhina, K.A. Rybakova, D.B. Davies, 1H NMR analysis of the complex formation of aromatic molecules of antibiotic and vitamin in aqueous solution: Heteroassociation of actinomycin D and flavin mononucleotide// Biophysics. -2005.-V. 50. - P. 20–27.
6. D.J. Patel, A.K. Suri. Structure, recognition and discrimination in RNA aptamer complexes with cofactors, amino acids, drugs and aminoglycoside antibiotics // J.Biotechnol. - 2000. - V. 74(1). - P. 39-60.
7. M.P. Evstigneev, Yu.V. Mukhina, D.B. Davies. Complexation of daunomycin with a DNA oligomer in the presence of an aromatic vitamin (B2) determined by NMR spectroscopy// Biophys. Chemist. -2005.-V. 118.-P. 118–127.
8. N. Dardare, M.S. Platz. Binding Affinities of Commonly Employed Sensitizers of Viral Inactivation// Photochemistry and Photobiology. – 2002. - V. 75(6). - P. 561–564.
9. K. Kuratomi, Y. Kobayashi. Studies on the interactions between DNA and flavins// Biochem. Biophys. Acta.- 1977. –V. 476.- P. 207–217.
10. Е.Г. Березняк, Н.А. Гладковская, Е.В. Духопельников, A.C. Хребтова, А.В. Зинченко. Особенности взаимодействия профлавина с ДНК при различных соотношениях их концентраций// Биофизика. -2009. – T. 54. - В.5. - C. 805-812.
11. F. Barcelо, D. Capо, J. Portugal. Thermodynamic characterization of the multivalent binding of chartreusin to DNA// Nucleic Acids Research. – 2002. - Vol. 30. - №. 20. - Р. 4567-4573.
12. H. Grajek, I. Gryczynski, P. Bojarski, Z. Gryczynski, Sh. Bharill, L. Kułak. Flavin mononucleotide fluorescence intensity decay in concentrated aqueous solutions// Chemical Physics Letters. – 2007. – V. 439. - Р. 151–156.
13. J. T. M. Kennis, S. Crosson, M. Gauden, I. H. M. van Stokkum, K. Moffat, R. van Grondelle Primary Reactions of the LOV2 Domain of Phototropin, a Plant Blue-Light Photoreceptor// Biochemistry. - 2003. - V. 42 (12). – Р. 3385-3392.
14. А.Т. Карапетян, П.О. Вардеванян, М.Д. Франк-Каменецкий. Влияние концентрации ионов на теплоту перехода спираль-клубок ДНК// Биополимеры и клетка.- 1989. - Т.5. - № 5. - С. 31-37.
15. A.T. Karapetian, P.O. Vardevanian, M.D. Frank-Kamentskii. Enthalpy of Helix-Coil Transition of DNA Depence on Na+ Concentration and GC Content// J. Biomol. Struct. Dyn. – 1990. - V. 8(1). - P. 131-138.
16. J.D.McGhee, P.H. von Hippel. Theoretical aspects of DNA-Protein interactions: Cooperative and non-cooperative binding of large ligands to a one-dimensional homogeneous lattice// J. Mol. Biol. - 1974. – V. 86. - P. 469-489.
17. J.B. Chaires. Energetics of drug-DNA interactions// Biopolymers.- 1997. – V. 44(3). - P. 201-15.
18. L.-P. Lin, L.-S. Huang, C.-W. Lin, C.-K. Lee, J.-L. Chen, S.-. Hsu, Sh. Lin. Determination of Binding Constant of DNA-binding Drug to Target DNA by Surface Plasmon Resonance Biosensor Technology// Current Drug Targets - Immune, Endocrine & Metabolic Disorders. - 2005. - V. 5. - P. 61-72.
Cited
How to Cite
Bereznyak, E. G., Gladkovskaya, N. A., Dukhopelnikov, E. V., Khrebtova, A. S., & Zinchenko, A. V. (1). Spectroscopic and calorimetris investigation of flavin-mononucleotide - DNA system. Biophysical Bulletin, 1(24). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/3865
Section
Molecular biophysics
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
