AgNPs Incorporated in PMMA-PVP blended nanofibers: spectroscopy monitoring of AgNP dissolution in PMMA nanopores
Abstract
Background: Electrospun polymeric nanofibers incorporated with some biologically active nanoparticles have a huge range of various applications in biomedical fields. Blending several polymers with different properties allows one to obtain a new material with improved characteristics, as well as to control the incorporation and release of medical agents.
Objectives: To elaborate an approach for the preparation of biocompatible nanofibers using a blend of two polymers (polyvinylpyrrolidone (PVP) and polymethyl methacrylate (PMMA)) with incorporated silver nanoparticles (AgNPs) and to apply the absorption spectroscopy for determining of the average nanoparticle diameter and monitoring of AgNP dissolution from PMMA nanopores.
Materials and methods: A blend of hydrophilic (PVP) and water-insoluble (PMMA) polymers is proposed for nanofiber preparation with incorporated AgNPs. The absorption peak position of the band due to localized surface plasmon resonance (LSPR) and its intensity in the UV-vis spectrum were used to characterize AgNPs and to estimate the influence of the environment.
Results: A new method for fabricating nanofibers from a mixture of two polymers, one water-soluble (PVP) and the other soluble only in organic solvents (PMMA), with AgNPs, has been developed. The diameter of the nanofibers is determined to be in the range of 2-4 μm. The average nanoparticle diameter determined by the position of the absorption peak due to LSPR in the UV-vis spectrum is 35-40 nm. The peak shift of this band in different environments was determined in comparison with the spectrum obtained in an aqueous solution. A decrease in the intensity of the band was observed with an increase in the mat incubation time in the oxidizing solution, and a blue shift of the band maximum was detected, which indicates a decrease in the average size of AgNPs during their dissolution.
Conclusions: In the blended nanofibers, PVP environment of AgNPs facilitates their incorporation into PMMA and provides access of water molecules to nanoparticles, while PMMA provides the mechanical strength of the nanofibers. The appearance of AgNPs from the polymeric nanofibers soaked in water was not detected, since they are fixed in the structure of the water-insoluble polymer. Nevertheless, the experiments with the mat soaking in the oxidative solution showed that the nanopores in PMMA incorporated with AgNPs are open. This observation indicates the possibility of a gradual release of Ag+ ions from such nanofibers.
Downloads
References
Abadi B, Goshtasbi N, Bolourian S, Tahsili J, Adeli-Sardou M, Forootanfar H. Electrospun hybrid nanofibers: Fabrication, characterization, and biomedical applications. Front Bioeng Biotechnol. 2022;10:986975. https://doi.org/10.3389/fbioe.2022.986975
Fromager B, Marhuenda E, Louis B, Bakalara N, Cambedouzou J, Cornu D. Recent advances in electrospun fibers for biological applications. Macromol. 2023;3:569–613. https://doi.org/10.3390/macromol3030033
Liu H, Gough CR, Deng Q, Gu Z, Wang F, Hu X. Recent advances in electrospun sustainable composites for biomedical, environmental, energy, and packaging applications. Int J Mol Sci. 2020;21:4019. https://doi.org/10.3390/ijms21114019
Dong Y, Zheng Y, Zhang K, Yao Y, Wang L, Li X, Yu J. Ding B. Electrospun nanofibrous materials for wound healing. Adv Fiber Mater. 2020;2:212–27. https://doi.org/10.1007/s42765-020-00034-y
Cleeton C, Keirouz A, Chen X, Radacsi N. Electrospun nanofibers for drug delivery and biosensing. ACS Biomater Sci Eng. 2019;5:4183−205. https://doi.org/10.1021/acsbiomaterials.9b00853
Keirouz A, Chung M, Kwon J, Fortunato G, Radacsi N. 2D and 3D electrospinning technologies for the fabrication of nanofibrous scaffolds for skin tissue engineering: A review. WIRES Nanomed Nanobiotechnol. 2020;12:e1626. https://doi.org/10.1002/wnan.1626
Plokhotnichenko AM, Karachevtsev VA. Electrospinning production of polymer nanofibers containing Ag nanoparticles or carbon nanotubes. Low Temp Phys. 2022;48:339–43. https://doi.org/10.1063/10.0009740
Huo P, Han X, Zhang W, Zhang J, Kumar P, Liu B. Electrospun Nanofibers of Polycaprolactone/Collagen as a Sustained-Release Drug Delivery System for Artemisinin. Pharmaceutics. 2021;13:1228. https://doi.org/10.3390/pharmaceutics13081228
Maliszewska I, Czapka T. Electrospun polymer nanofibers with antimicrobial activity. Polymers. 2022;14:1661. https://doi.org/10.3390/polym14091661
Zupančič S, Sinha-Ray Sumit, Sinha-Ray Suman, Kristl J, Yarin AL. Controlled release of ciprofloxacin from core-shell nanofibers with monolithic or blended core. Mol Pharm. 2016;13:1393–404. https://doi.org/10.1021/acs.molpharmaceut.6b00039
Zupančič S, Sinha-Ray Sumit, Sinha-Ray Suman, Kristl J, Yarin AL. Long-term sustained ciprofloxacin release from pmma and hydrophilic polymer blended nanofibers. Mol Pharm. 2016;13:295–305. https://doi.org/10.1021/acs.molpharmaceut.5b00804
Virginia C, Khasanah A, Jauhari J, Sriyanti I. Electrospinning and characterization nanofibers and nanoparticle of Polyvinylpyrrolidone. IOP Conf. Series: Materials Science and Engineering. 2020;50:012039. https://doi.org/10.1088/1757-899X/850/1/012039
Restrepo CV, Villa CC. Synthesis of silver nanoparticles, influence of capping agents, and dependence on size and shape: A review. Environ Nanotechnol Monit Manag. 2021;15:100428. https://doi.org/10.1016/j.enmm.2021.100428
Liao C, Li Y, Tjong SC. Bactericidal and Cytotoxic Properties of Silver Nanoparticles. Int J Mol Sci. 2019;20:449. https://doi.org/10.3390/ijms20020449
Wang H, Qiao X, Chen J, Wang X, Ding S. Mechanisms of PVP in the preparation of silver nanoparticles. Mat Chem Phys. 2005;94:449–53. https://doi.org/10.1016/j.matchemphys.2005.05.005
Khanlou HM, Ang BC, Talebian S, Afifi AM, Andriyana A. Electrospinning of polymethyl methacrylate nanofibers: optimization of processing parameters using the Taguchi design of experiments. Text Res J. 2015;85:356–68. https://doi.org/10.1177/0040517514547208
Piperno S, Lozzi L, Rastelli R, Passacantando M, Santucci S. PMMA nanofibers production by electrospinning. Appl Surf Sci. 2006;252:5583–6. https://doi.org/10.1016/j.apsusc.2005.12.142
Wang H, Liu Q, Yang Q, Li Y, Wang W, Sun L, Zhang C, Li Y. Electrospun poly(methyl methacrylate) nanofibers and microparticles. J Mater Sci. 2010;45:1032–8. https://doi.org/10.1007/s10853-009-4035-1
Macossay J, Marruffo A, Rincon R, Eubanks T, Kuang A. Effect of needle diameter on nanofiber diameter and thermal properties of electrospun poly(methyl methacrylate). Polym Adv Technol. 2007;18:180–3. https://doi.org/10.1002/pat.844
Paramelle D, Sadovoy A, Gorelik S, Free P, Hobleya J, Fernig DG. A rapid method to estimate the concentration of citrate capped silver nanoparticles from UV-visible light spectra. Analyst. 2014;139:4855–61. https://doi.org/10.1039/C4AN00978A
Willets KA, Van Duyne RP. Localized surface plasmon resonance spectroscopy and sensing. Annu Rev Phys Chem. 2007;58:267–97. https://doi.org/10.1146/annurev.physchem.58.032806.104607
Copyright (c) 2025 Alexander Plokhotnichenko, Victor Karachevtsev
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
