INFLUENCE OF γ-RADIATION ON STRUCTURAL STATE OF DNA IRRADIATED AT VARIOUS HUMIDITY LEVELS

  • А. А. Krasnitskaya Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine
  • A. І. Gasan Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine
  • К. М. Virnik Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine
Keywords: DNA

Abstract

A study of γ-radiation influence on calf thymus NaDNA at various relative humidity levels has been carried out with using gel-electrophoresis and UV-spectroscopy techniques. It has been shown that irradiated DNA samples differ from check sample in molecular mass (fragment lengths) distribution due to partial fragmentation of DNA molecules, the fragmentation degree depending upon relative humidity of the sample during irradiation. The most destruction of DNA is observed at minimum (0%) and maximum (90%) relative humidity, and the least one - in the interval of 56-64% relative humidity. UV-spectroscopy data give evidence of that with increase of relative humidity of DNA samples irradiated with a certain dose shift of DNA melting temperature towards its increase is observed. It is concluded that main targets of y-radiation influence on DNA are diesteric bonds between AT-pairs.

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Author Biographies

А. А. Krasnitskaya, Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine

12 Acad. Proskura str., Kharkov 310085, Ukraine

A. І. Gasan, Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine

 12 Acad. Proskura str., Kharkov 310085, Ukraine

К. М. Virnik, Institute of Radiophysics and Electronics, National Academy of Sciences of Ukraine

12 Acad. Proskura str., Kharkov 310085, Ukraine

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How to Cite
KrasnitskayaА. А., GasanA. І., & VirnikК. М. (1). INFLUENCE OF γ-RADIATION ON STRUCTURAL STATE OF DNA IRRADIATED AT VARIOUS HUMIDITY LEVELS. Biophysical Bulletin, 1(1). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2067
Section
Action of physical agents on biological objects