INFLUENCE OF γ-RADIATION ON STRUCTURAL STATE OF DNA IRRADIATED AT VARIOUS HUMIDITY LEVELS
Abstract
A study of γ-radiation influence on calf thymus NaDNA at various relative humidity levels has been carried out with using gel-electrophoresis and UV-spectroscopy techniques. It has been shown that irradiated DNA samples differ from check sample in molecular mass (fragment lengths) distribution due to partial fragmentation of DNA molecules, the fragmentation degree depending upon relative humidity of the sample during irradiation. The most destruction of DNA is observed at minimum (0%) and maximum (90%) relative humidity, and the least one - in the interval of 56-64% relative humidity. UV-spectroscopy data give evidence of that with increase of relative humidity of DNA samples irradiated with a certain dose shift of DNA melting temperature towards its increase is observed. It is concluded that main targets of y-radiation influence on DNA are diesteric bonds between AT-pairs.
Downloads
References
2. Тимофеев-Ресовский Н. В., Савич А. В., Шальное М. И. Введение в молекулярную радиобиологию. М. Медицина, 1981. 320с.
3. Радиационная биофизика ДНП хроматина. М. Атомиздат, 1978. 224с.
4. Первичные радиобиологические процессы. Под ред. Тимофеева-Ресовского. М. Атомиздат, 1972. 336с.
5. Кузин А. Н. Радиационная биохимия. М. Изд-во АН СССР, 1982. 346 с.
6. Swarts S.G., Sevilla M.D., Becker D., Tokar C.J , Wheeler K.T. // Radiation Research. 1992. V.129. P.333-344.
7. Becker D., La Vere Т., Sevilla M.D. // Radiation Research. 1994. V. 140. P. 123-129.
8. La Vere Т., Becker D., Sevilla M.D. // Radiation Research. 1996. V.145. P.673-680.
9. Ohshima H, Iida Y.. Matsuda A., Kuwabara M. // Journal of Radiation Research. 1996. V.37. P. 199-207.
10. Swarts S.G., Becker D., Sevilla M., Wheeler K.T. // Radiation Research. 1996. V.145. P.304-314.
11. Малеев В.Я., Семенов M.A., Гасан А.И., Кашпур В.А. // Биофизика. 1993. Т.38. С.768-790.
12. Lindsay S. М, Lee S. A., Powell J. М., Weidlich Т., DeMarko С, Lewen G. D., Tao N. J., & Rupprecht A. // Biopolymers. 1989. V.27. P.1015-1043.
13. Воронец Д., Козин Д. Влажный воздух: термодинамические свойства и применение. М. Энергоатомиздат, 1984. 136с.
14. Hutterraann J., Rohrig М., Kohnlein W. // International Journal of Radiation Biology. 1992. V.61. P.299-313.
15. Yen W. S, Blake R. D. //Biopolymers. 1980. V.19. P.681-700.
16. Marmur J., Doty P. // J. Моl. Biol. 1962. V.5. P.109-118.
17. Франк-Каменецкий М. Д., Франк-Каменецкий А.Д. // Молекулярная биология. 1968. Т.2. С.778-783.
18. Берестецкая И.В., Косаганов Ю.Н., Лазуркин Ю.С., Трифонов Э.Н. и Франк-Каменецкий М. Д. //Молекулярная биология. 1970. Т.4. С.137-143.
19. Волькенштейн М.В. Молекулярная биофизика. М. Наука, 1975. 616с.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
