INFLUENCE OF γ-IRRADIATION ON STATE OF DNA ION-HYDRATION SHELL
Keywords:
DNA, electrophoresis
Abstract
The effect of γ-radiation on the state of DNA hydration shell has been investigated. The changes of dielectric permittivity of DNA aqueous solutions irradiated at doses 19, 370, and 1650 Gy have been studied using the differential dielectrometric method. The DNA state was checked by conductance-measuring and electrophoresis methods. Some noticeable changes of DNA hydration at 370 and 1650 Gy have been found. It has been shown that the effects revealed are due to the breach of water backbone structure arising from double helix reconstructions which appear to be a result of radiation deoxyribosyl lesions and also to the appearance of DNA radiation-altered bases.
Downloads
Download data is not yet available.
References
Рябченко Н.И. Радиация и ДНК. М. Атомиздат, 1979. 192с.
Шарпатый В.А. // Радиобиология. 1992. Т.32. С.180-193.
Зенгер В. Принципы структурной организации нуклеиновых кислот. М. Мир, 1987. 584с. .
Swarts S.G., Sevilla M.D., Becker D., Tokar C.J.. Wheeler K.T. // Radiat. Res. 1992. V.129. P.333-344.
Pankowski E. // Studia biophys. 1988. V.128. P.27-36.
Остерман Л.А. Методы исследования белков и нуклеиновых кислот. М. Наука, 1981. 286с.
Кашпур В.А., Малеев В.Я., Щеголева Т.Ю. // Мол. биол. 1976. Т. 10. С.568-575.
Maleev V.Ya., Kashpur V.A., Glibitsky G.M., Krasnitskaya A.A., Veretelnik Ye.V. // Biopolymers. 1987. V.26. P. 1965-1970.
Adinarayana M., Bothe E., Schulte-Frohlinde D. // Int. J. Radiat. Biol. 1988. V.54. P.723-733.
Umehara T, Kuwabara S., Mashimo S., Yagihara S. // Biopolymers. 1990. V.30. P.649-656.
Trumbore C. N., Myers Y. N., Hyde C.K., Hudson R.D., Rhodes C.N., Masselink J.K. // Int. J. Radiat. Biol. 1994. V. 66. P.479-483.
Collyns В., Okada S., Scholes G, Weiss J.J., Wheeler C.M. // Radiat. Res. 1965. V. 25. P.526-536.
Fuciarelli A.F., Wegher B.J., Blakely W.F., Dizdaroglu M. // Int. J. Radiat. Biol. 1990. V.58. P.397-415.
Dizdaroglu M. // Int. J. Radiat. Biol. 1992. V. 62. P. 175-183.
Henle E.S., Roots R, Holley W.R., Chatterjee A. // Radiat. Res. 1995. V. 143. P. 144-150.
Шарпатый В.А. // Радиобиология. 1992. Т.32. С.180-193.
Зенгер В. Принципы структурной организации нуклеиновых кислот. М. Мир, 1987. 584с. .
Swarts S.G., Sevilla M.D., Becker D., Tokar C.J.. Wheeler K.T. // Radiat. Res. 1992. V.129. P.333-344.
Pankowski E. // Studia biophys. 1988. V.128. P.27-36.
Остерман Л.А. Методы исследования белков и нуклеиновых кислот. М. Наука, 1981. 286с.
Кашпур В.А., Малеев В.Я., Щеголева Т.Ю. // Мол. биол. 1976. Т. 10. С.568-575.
Maleev V.Ya., Kashpur V.A., Glibitsky G.M., Krasnitskaya A.A., Veretelnik Ye.V. // Biopolymers. 1987. V.26. P. 1965-1970.
Adinarayana M., Bothe E., Schulte-Frohlinde D. // Int. J. Radiat. Biol. 1988. V.54. P.723-733.
Umehara T, Kuwabara S., Mashimo S., Yagihara S. // Biopolymers. 1990. V.30. P.649-656.
Trumbore C. N., Myers Y. N., Hyde C.K., Hudson R.D., Rhodes C.N., Masselink J.K. // Int. J. Radiat. Biol. 1994. V. 66. P.479-483.
Collyns В., Okada S., Scholes G, Weiss J.J., Wheeler C.M. // Radiat. Res. 1965. V. 25. P.526-536.
Fuciarelli A.F., Wegher B.J., Blakely W.F., Dizdaroglu M. // Int. J. Radiat. Biol. 1990. V.58. P.397-415.
Dizdaroglu M. // Int. J. Radiat. Biol. 1992. V. 62. P. 175-183.
Henle E.S., Roots R, Holley W.R., Chatterjee A. // Radiat. Res. 1995. V. 143. P. 144-150.
Cited
How to Cite
KashpurV. А., DubovitskayaО. V., KrasnitskayaА. А., & Maleev, V. Y. (1). INFLUENCE OF γ-IRRADIATION ON STATE OF DNA ION-HYDRATION SHELL. Biophysical Bulletin, 1(1). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2059
Section
Action of physical agents on biological objects
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
