Permeability of rat hepatocyte plasma membrane for water molecule in non-isotonic medium of electrolyte

  • L.G.Kuleshova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine
  • E.A.Gordienko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine
  • I.F.Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine
Keywords: hepatocytes, membrane, permeability, osmolarity

Abstract

Rat hepatocyte osmotic behavior in non-isotonic medium of electrolyte (NaCl) was studied using the method of light microscopy. Cell dehydration kinetic curves obtained analytically by means of nonequilibrium thermodynamics equations, were used to calculate numerical values of hepatocyte membrane filtration coefficient for water molecule (Lp) and a volumetric part of osmotically non-active intracellular substances as well. It was established, that the filtration coefficient L depends on the electrolyte concentration in the medium and decreased from 2,17 · 10-11 m3/Hc down to 0,50 · 10-11 m3/Hc when increasing an extracellular osmotic pressure rate from 1087 mOsm up to 6636 mOsm respectively.

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Author Biographies

L.G.Kuleshova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine

23 Pereyaslavskaya st., 61015, Kharkov, Ukraine

E.A.Gordienko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine

23 Pereyaslavskaya st., 61015, Kharkov, Ukraine

I.F.Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine

23 Pereyaslavskaya st., 61015, Kharkov, Ukraine

References

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2. Кравченко Л.П., Андриенко А Н., Семенченко О.А., Петренко А.Ю., Сукач А.Н., Способ получения изолированных клеток печени А.с.№1463757, С12N5/00, з.№4221432/28-13 от 02.04.87. опубл. 07.03 89, БИ №9

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Published
2002-06-05
Cited
How to Cite
Kuleshova, L., Gordienko, E., & Kovalenko, I. (2002). Permeability of rat hepatocyte plasma membrane for water molecule in non-isotonic medium of electrolyte. Biophysical Bulletin, 2(11), 39-42. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/18510
Issue
Vol. 2 No. 11 (2002): Біофізичний вісник
Section
Cell biophysics

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