Fluorescent probe study of model phosphatidylcholine/cardiolipin membranes

  • E.S. Zhilkova V.N. Karazin Kharkov National University
  • G.P. Gorbenko V.N. Karazin Kharkov National University
  • Ye.A. Domanov V.N. Karazin Kharkov National University
Keywords: cardiolipin, liposomes, fluorescent probes, bilayer structure

Abstract

The effect of negatively charged phospholipid cardiolipin (CL) on the structural state of phosphatidylcholine (PC) model membranes was studied using fluorescent probes 4-(n-dimethylaminostyryl)-1-methylpyridinium (DSM) and 3 methoxybenzanthrone (MBA). Electrostatic and non-electrostatic contributions to the stabilization of DSM-lipid complexes were evaluated. MBA bilayer distribution was found to change on increasing CL content from 5 to 10 mol % suggesting that incorporation of small amounts of CL into PC bilayer is followed by pronounced modification of the membrane structure

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Author Biographies

E.S. Zhilkova, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077, Ukraine

G.P. Gorbenko, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077, Ukraine

Ye.A. Domanov, V.N. Karazin Kharkov National University

4 Svobody Sq., Kharkov, 61077, Ukraine

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Published
2005-06-06
Cited
How to Cite
Zhilkova, E., Gorbenko, G., & Domanov, Y. (2005). Fluorescent probe study of model phosphatidylcholine/cardiolipin membranes. Biophysical Bulletin, 1(15), 108-110. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/18290
Section
Methods of biophysical investigations