Potentialities of secondary emission mass spectrometry in control of immobilization of dyes in lipid layers and liposomes
Keywords:
lipids, dyes, immobilization, secondary ion mass spectrometry
Abstract
In the framework of a problem of immobilization of molecular components of biosensors, a mass spectrometric method of control of immobilization of dyes in lipid layer and liposomes is suggested. A criterion of successful immobilization of dyes in a lipid monolayer on a liquid matrix surface is substantiated, which consists in the simultaneous presence of the peaks corresponding to the lipids and the dyes in the secondary ion mass spectra of the system. A method of lipids identification by their low mass characteristic fragments, which can be applied using affordable analytical instruments with a limited mass range, is suggested.
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References
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9. Benninghoven A. // Int. J. Mass Spectrom Ion Phys. 1983. V. 53. P. 85-99.
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16. Maкитрук B.Л, Шаламай А.С., Кондратюк И.В.//Биополимеры и клетка.1997. N 13. C. 453-459.
17. Kosevich M.V., Boryak O.A., Orlov V.V., Shelkovsky V.S., Chagovets V.V., Stepanian S.G., Karachevtsev V.A., Adamowisz L. // J. Mass Spectrom. 2006. V. 41. N 1 P.13-123.
18. Bentz B.L., Gale P.J. // Int. J Mass Spectrom. Ion Proc. 1987. V. 78. P. 115-130.
19. Gale P.J., Bentz B.I., Chait B.T., Field F.H., Cotter R.J. // Analytical Chemistry. I986. V. 58. P. 1070-1076
20. Kyranos J.N., Vouros P. // Biomed. Envir. Mass Spectrom. 1990. V. 19 P. 628-634.
21. Ohashi Y., Itoh Y. // Current Organic Chemistry. 2003. V. 7. P. 1605-1611
22. Pelzer G., De Pauw E., Dao V.D., Marient J. // J. Phys. Chem.. 1984 V. 88 P. 5065-5068
23. Cerny R.L., Gross M.L. // Analytical Chemistry. 1985. V 57. P. 1160-1163.
24. Rvazanova O.A., Zozulya V.N., Voloshin I.M., Karachevtsev V.A., Makitruk V.L., Stepanian S.G. // Spectrochimica Acta. Part A. Molecular and Biomolecular Spectroscopy. 2004. V. 60 P. 2005-2011.
25. Liu P., Finashin A.V., Domanov Ye. A., Gorbenko G.P. // Visnyk Kharkivs'koho Natsional'noho Universytetu im.V.N.Karazina. Biophysical Bulletin. 2005 (in press)
Published
2005-06-06
Cited
How to Cite
O.A. Boryak, O., Kosevich , M., Chagovets, V., Shelkovsky, V., Orlov, V., Karachevtsev, V., G.P. Gorbenko, G., Domanov, Y., & Tovstiak, V. (2005). Potentialities of secondary emission mass spectrometry in control of immobilization of dyes in lipid layers and liposomes . Biophysical Bulletin, 2(16), 97-105. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/17940
Section
Methods of biophysical investigations
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