Fractal textures of bovine serum albumin films
Keywords:
solution, film, albumin, fractal, texture
Abstract
The texture features of the films obtained from solutions of bovine serum albumin (BSA) in the presence
of sodium and copper ions are considered. The apparatus for producing films and the absorption spectra
of BSA in the UV wavelength range are presented. The films have been received from BSA solutions
with concentration of 0.41 mg/ml in the presence of salt NaCl at a concentration of 20 mM and in the
presence of salt CuCl2 with concentrations of 0.1 mM and 0.4 mM. Texture films of BSA, human serum
albumin and calf thymus DNA are compared. The obtained scale invariant fractal textures are similar to
textures which are formed on the films surface from the DNA solutions. Presence in solution of heavy
metal ions forming compounds with albumin molecules prevents the formation of scale invariant fractal
textures on films from these solutions.
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References
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2. Швецова Н.А. Диагностика заболеваний по картине самоорганизации в высыхающих каплях биологических жидкостей / Н.А. Швецова // Биомедицинская радиоэлектроника. – 2012. – №3. – С. 30-36. /Shvecova N.A. Diagnostika zabolevanij po kartine samoorganizacii v vysyhajushhih kapljah biologicheskih zhidkostej / N.A. Shvecova // Biomedicinskaja radiojelektronika. – 2012. – №3. – S. 30-36./
3. Шатохина С. Н. Маркер дискогенных заболеваний нервной системы в цереброспинальной жидкости / Н. Шатохина, В. С. Кузнецова, В. Н. Шабалин // Журнал неврологии и психиатрии им.С.С.Корсакова. – 2011. – № 12. С. 4–8. /Shatohina S. N. Marker diskogennyh zabolevanij nervnoj sistemy v cerebrospinal'noj zhidkosti / N. Shatohina, V. S. Kuznecova, V. N. Shabalin // Zhurnal nevrologii i psihiatrii im.S.S.Korsakova. – 2011. – № 12. S. 4–8./
4. Maksimov S. A. Morphology of the solid phase of biological liquids as method of diagnostics in medicine / S. A. Maksimov // Bulletin of Siberian medicine. – 2007. – № 4. – P.80–83.
5. Тарасевич Ю. Ю. Механизмы и модели дегидратационной самоорганизации биологи¬ческих жидкостей / Ю. Ю. Тарасевич // Успехи физических наук. – 2004. – №7. – С. 779–790. /Tarasevich Ju. Ju. Mehanizmy i modeli degidratacionnoj samoorganizacii biologi¬cheskih zhidkostej / Ju. Ju. Tarasevich // Uspehi fizicheskih nauk. – 2004. – №7. – S. 779–790./
6. Белок и соль: пространственно-временные события в высыхающей капле / Т. А., Яхно, В. Г. Яхно, А. Г. Санин [и др.] // ЖТФ. – 2004. – №8. – С. 100–108. /Belok i sol': prostranstvenno-vremennye sobytija v vysyhajushhej kaple / T. A., Jahno, V. G. Jahno, A. G. Sanin [i dr.] // ZhTF. – 2004. – №8. – S. 100–108./
7. Tarasevich Y. Y. Modeling of spatial-temporal distribution of the components in the drying sessile droplet of biological fluid / Y. Y. Tarasevich, I. V. Vodolazskaya, O. P. Bondarenko // Colloids and Surfaces A: Physicochemical and Engineering Aspects – 2013. Vol. 432. – P. 99–103.
8. A study of Na-DNA films containing NaCl via scanning electron and tunneling microscopies / C. H. Mayeres, S. A. Lee, D. A. Pinnick [et al] // Biopolymers. – 1995. – Vol. 36. – P. 669–673.
9. Perepelytsya S. M. Texture formation in DNA films with alkali metal chlorides / S. M. Perepelytsya, G. M. Glibitskiy, S.N. Volkov // Biopolymers. – 2013. Vol. 99. – P. 508–16.
10. Глибицкий Г. М. Текстуры пленок Na-ДНК, полученные из растворов, содержащих ТРИС и ЭДТА / Г. М. Глибицкий, М. А. Семенов, Д. М. Глибицкий // Біофізичний вісник – 2011. – №2. – С. 26–30. /Glibickij G. M. Tekstury plenok Na-DNK, poluchennye iz rastvorov, soderzhashhih TRIS i JeDTA / G. M. Glibickij, M. A. Semenov, D. M. Glibickij // Bіofіzichnij vіsnik – 2011. – №2. – S. 26–30./
11. Glibitskiy G. M. Interaction of DNA with Silver Nanoparticles / G. M. Glibitskiy, V. V. Jelali, M. O. Semenov [et al.] // Ukr. J. Phys. – 2012. – Vol. 7. – P.695–699.
12. Glibitskiy G. М. Structures of Na-DNA films received from solutions at various values of humidity and temperatures / G. М. Glibitskiy, A. A. Krasnitskaja, V. I. Gudzenko // Biophysical Bulletin. – 2006. –Vol. 1. – P. 89–94.
13. Степанченко Н. С. Количественное определение содержания белка / Н. С. Степанченко, Г. В. Новикова, И. Е. Мошков // Физиология растений. – 2011. – № 4. – C.624–630. /Stepanchenko N. S. Kolichestvennoe opredelenie soderzhanija belka / N. S. Stepanchenko, G. V. Novikova, I. E. Moshkov // Fiziologija rastenij. – 2011. – № 4. – C.624–630./
14. Glibitskiy G. M. // Nanoboiphysics: “Fundamental and Applied Aspects. Third International Conference.” – Kharkiv:- Publishing “B.Verkin Institute for Low Temperature Physics and Engineering,” 2013. – P.109.
15. Kotz J. Self-assembled polyelectrolyte systems / J. Kotz, S. Kosmella, T. Beitz. // Prog. Polym. Sei. – 2001. Vol. 26. – P. 1199–1232.
16. Peters T. Copper-binding Properties of Bovine Serum Albumin and Its Amino-terminal Peptide Fragment / T. Peters, F. A. Blumenstock // Journ. Biol. Chem. – 1967. – Vol. 242. – P. 1574–1578.
17. Impact of copper to the chelation effect of bovine serum albumin and spermatozoa motility parameter in vitro / Z. Kňažická, J. Lukáčová, E. Tvrdá [et al] //Contemporary Agriculture, 2012. Vol. 61. – P. 15–28.
18. Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin / M. Sokolowska, A. Krezel, I. Dyba [et al] / Eur. J. Biochem. – 2002. – Vol. 269. – P. 1323–1331.
19. Farruggia B. G. Pico. Bile Salts Binding-Induced Alterations in Ultraviolet Absorption Spectrum of Bovine Serum Albumin / B. Farruggia, G. Pico // Gen. Physiol. Biophys. – 1992. – Vol. 11. – P. 4–218.
20. Glibitskiy G.M., Krasnitskaya A.A. Authors’ certificate N 86246 UA, Bull. N.7. (10.04.2009).
Cited
How to Cite
Glibitskiy, G. G. (1). Fractal textures of bovine serum albumin films. Biophysical Bulletin, 2(30). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/1595
Section
Molecular biophysics
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