Investigation of the effect of covalently attached phenazine dye on helix-to-coil transition in mixed poly (rA)-(dT)14 system

  • О. А. Ryazanova Verkin Institute for Low Temperature Physics and Engineering of NAS of Ukraine
  • I. Ya. Dubey Institute of Molecular Biology and Genetics of NAS of Ukraine
  • V. N. Zozulya Verkin Institute for Low Temperature Physics and Engineering of NAS of Ukraine
Keywords: thermal denaturation, UV-absorption, fluorescent spectroscopy, helix-to-coil transition, poly(rA), (dT)14, imidazophenazine

Abstract

The effect of imidazo-(4,5-d)-phenazine-N1-β-D-ribofuranoside (Pzn) covalently attached to 3'-end of (dT)14 on conformational transition in a mixed duplex system of poly(rA)•(dT)14 was investigated. The measurements were carried out in Na-cacodylate buffered solution, pH 6.9, containing 0.1 М NaCl, with polynucleotide concentration of 10-300 mkM base pairs. Formation of the poly(rA) complexes with (dT)14 and (dT)14Pzn was studied by thermal denaturation method based on the registration of melting curves by changes in the UV-absorption and the fluorescence intensity of attached phenazine dye. It was found that covalent attachment of imidazophenazin nucleoside derivative substantially increases the thermostability of duplexes formed by (dT)14 with the poly(rA), increasing the temperature of the helix-to-coil transition of 7°С due to intercalation of the dye chromophore between adenine bases. The thermodynamic parameters of helix-to-coil transition for systems studied were determined using the "all-or-none" model. It was shown, that the dye conjugation results in a decrease in the transition enthalpy. However, the observed gain in the free energy of complex formation, ΔG, is provided with the corresponding decrease in entropy change

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Author Biographies

О. А. Ryazanova, Verkin Institute for Low Temperature Physics and Engineering of NAS of Ukraine

47 Lenin ave., 61103, Kharkiv

е-mail: гуаzапоva@ilt.кhагкоv.uа

I. Ya. Dubey, Institute of Molecular Biology and Genetics of NAS of Ukraine

150 Zabolotnogo str., 03143, Kiev

е-mail: гуаzапоva@ilt.кhагкоv.uа

 

V. N. Zozulya, Verkin Institute for Low Temperature Physics and Engineering of NAS of Ukraine

47 Lenin ave., 61103, Kharkiv

е-mail: гуаzапоva@ilt.кhагкоv.uа

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Published
2008-06-03
Cited
How to Cite
RyazanovaО. А., Dubey, I. Y., & Zozulya, V. N. (2008). Investigation of the effect of covalently attached phenazine dye on helix-to-coil transition in mixed poly (rA)-(dT)14 system. Biophysical Bulletin, 1(20), 17-23. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/1536