Change of different haemoglobin form content in solution after heating

  • P. V. Voloshin Kharkov State University of Technology and Management of Nutrition
  • A. I. Cherevko Kharkov State University of Technology and Management of Nutrition
  • E. D. Rozanova Kharkov State University of Technology and Management of Nutrition
  • V. A. Kovalenko Kharkov State University of Technology and Management of Nutrition
  • V. V. Yevlash Kharkov State University of Technology and Management of Nutrition
Keywords: heamoglobin, Fe2 , heating, spectrophotometry, ascorbate, lysine, sacharose

Abstract

The effect of ascorbat, lysin and sacharose addition to the solution on oxigenation of haem iron was studied. Concentration of different haemoglobin forms was measured spectrofotometrically. Dramatical decrease of methaemoglobin fromation under heating was established in presence of sacharose.

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Author Biographies

P. V. Voloshin, Kharkov State University of Technology and Management of Nutrition

333 Klochkovskaya St., Kharkov 61000

A. I. Cherevko, Kharkov State University of Technology and Management of Nutrition

333 Klochkovskaya St., Kharkov 61000

E. D. Rozanova, Kharkov State University of Technology and Management of Nutrition

333 Klochkovskaya St., Kharkov 61000

V. A. Kovalenko, Kharkov State University of Technology and Management of Nutrition

333 Klochkovskaya St., Kharkov 61000

V. V. Yevlash, Kharkov State University of Technology and Management of Nutrition

333 Klochkovskaya St., Kharkov 61000

References

Gorbatov VM, Mamonov ND. Obrabotka i ispolzovanie krovi na pishchivye tceli.TcNIIGEN miasoprom; 1985. 20p. (in Russian)

Cherniaev SI, Liublinskii SL, Liublinskaia IN, Markov NV. "Gemoglobin" - naturalnaia biologicheski aktivnaia pishchevaia dobavka novogo pokoleniia. Pishchevaia promyshlennost. 2000;8:50-2. (in Russian)

Lapshina EA, Zavodnik IB, Ignatenko VA, Stepuro II. Termostabilnost i funktcionalnye svoistva gemoglobina cheloveka v prisutstvii alifaticheskikh spirtov. Mol. Biol. 1992;26(2):315-20. (in Russian)

Ernst R, Jaffe MD. Methemoglobin Pathology in the function of red blood cells:eritrocite pathology. NY. 1981. 133-151.

Aleksandrov VIa. Kletki, makromolekuly i temperatura. L.: Nauka; 1975. 329p. (in Russian)

Zwart A, Buursuia A,van Kampen EJ, Desburg B, van der Ploeg PHW, Zilistra WG. A multi-wavelength Spectrophtometric Method for the simultaneous determination of five Haemoglobin derivatives. J. Clin Chem. Clin. Biochem. 1981;19:457-63.

Overwie of blood, Ed.C.Bishop; 1978. 431p.

Uait A, Khendler F, Smit E. Osnovy biokhimii. Moskva:Mir; 1981. 726p. (in Russian)
Published
2018-06-25
Cited
How to Cite
Voloshin, P. V., Cherevko, A. I., Rozanova, E. D., Kovalenko, V. A., & Yevlash, V. V. (2018). Change of different haemoglobin form content in solution after heating. Biophysical Bulletin, 2(13), 100-103. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12455
Section
Action of physical agents on biological objects